• Login
    View Item 
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA CatalogsUA Libraries

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    An evaluation of bacterial gene expression during the biodegradation of organic contaminants

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    azu_td_9923160_sip1_c.pdf
    Size:
    6.874Mb
    Format:
    PDF
    Download
    Author
    Marlowe, Elizabeth Mary
    Issue Date
    1999
    Keywords
    Biology, Molecular.
    Biology, Genetics.
    Biology, Microbiology.
    Environmental Sciences.
    Advisor
    Pepper, Ian L.
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    The use of molecular techniques to examine remediation sites has primarily been used to track bacterial genetic potential and evaluate microbial diversity. Characterization of genetic potential, however, only indicates that the gene which encodes for a specific enzyme is present in a sample. It fails to give any information as to the expression of the degradative gene. Although specific microbial activity is a direct result of gene expression of the degradative gene, little is known about the expression of degradative genes in the environment. The objective of this study was to develop methodology to evaluate specific microbial gene expression to apply to understanding the biodegradation of organic contaminants. The model system used in this study was Pseudomona putida G7 (PpG7) which contains the NAH7 plasmid that encodes for the genes involved in the degradation of polyaromatic hydrocarbons (PAH) such as naphthalene. A qualitative RT-PCR assay was developed and used to evaluate gene expression as an index of biodegradation. Gene expression was correlated with substrate disappearance and degradation kinetics. This assay was used to evaluate the influence of two solubilizing agents, known to increase the biodegradation and bioavailablity of phenanthrene, to determine their influence on microbial gene expression. Gene expression patterns correlated with the presence of the solubilizing agents, indicating increased cellular activity due to increased bioavailability of the substrate. This assay was also used to evaluate gene expression and divergence of the nahAc genotype. Three Pseudomonas spp., isolated from a contaminated site, were compared to the well-studied PpG7 isolate. Although gene expression patterns correlated with degradation, the gene expression patterns of the three Pseudomonas sp. isolates were different from that of PpG7. These data suggest that gene expression is regulation-system dependent. Phylogenetic analysis of the nahAc genotype demonstrated that the Pseudomonas spp. were more closely related to another well studied naphthalene degrader, P. putida NCIB 9816, than to PpG7. These results demonstrated that gene expression can be used as an indicator of bioavailability and that divergence within a genotype is a factor in gene expression.
    Type
    text
    Dissertation-Reproduction (electronic)
    Degree Name
    Ph.D.
    Degree Level
    doctoral
    Degree Program
    Graduate College
    Soil, Water and Environmental Science
    Degree Grantor
    University of Arizona
    Collections
    Dissertations

    entitlement

     
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.