The synthesis and characterization of two-photon fluorescent chromophores for biological imaging
AuthorLavin, Judith M.
AdvisorMarder, Seth R.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractTwo consequences of two-photon absorption phenomena (TPA); pin-point resolution and improved depth penetration, have generated interest in its potential use in biological imaging. Conventional dyes used for confocal scanning laser microscopy work well for this one-photon absorption process, however have not been optimized for two-photon absorption. Thus a need exists for the synthesis of two-photon fluorescent dyes with a large two-photon absorption cross sections. A variety of TPA dyes, of three classes were synthesized and characterized: (i) bis(styryl)benzene derivatives; (ii) amphiphilic dyes; (iii) cyanine-like dyes. In the design of these dyes, the structural features that affected two-photon cross section delta, and the fluorescence quantum yield phifl, were chosen and tuned with the aim of optimizing these parameters. With the cyanine-like derivatives, the effects of resonance enhancement and detuning were also factors that required optimization through structural alterations and modification.
Degree ProgramGraduate College