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dc.contributor.advisorMarmorstein, Alan
dc.contributor.authorCross, Samuel David
dc.creatorCross, Samuel Daviden_US
dc.date.accessioned2013-08-07T22:03:29Z
dc.date.available2013-08-07T22:03:29Z
dc.date.issued2013
dc.identifier.urihttp://hdl.handle.net/10150/297532
dc.description.abstractThe planarian model is becoming an increasingly recognized model for tissue regeneration and embryonic development. In time, it is hoped that the investigation of the mechanisms of planarian tissue regeneration will provide a simple pathway by which complex patterns of tissue can be formed from very few stem cells. This has the potential to revolutionize the treatment of problems with early embryonic development, and because the planarian has no problem regenerating its entire central nervous system, it could also potentially lead to new treatments for neurodegenerative diseases. In this article, an overview for basic care and maintenance procedures for the planarian species Schmidtea mediterranea is provided. In addition, protocols for two commonly used techniques are provided: RNAi silencing of specific genes and the use of whole-mount in situ hybridization to sensitively visualize gene expression.
dc.language.isoenen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.titleProtocol for Maintaining a Colony of Schmidtea Mediterraneaen_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplinePhysiologyen_US
thesis.degree.nameB.S.H.S.en_US
refterms.dateFOA2018-06-17T16:07:09Z
html.description.abstractThe planarian model is becoming an increasingly recognized model for tissue regeneration and embryonic development. In time, it is hoped that the investigation of the mechanisms of planarian tissue regeneration will provide a simple pathway by which complex patterns of tissue can be formed from very few stem cells. This has the potential to revolutionize the treatment of problems with early embryonic development, and because the planarian has no problem regenerating its entire central nervous system, it could also potentially lead to new treatments for neurodegenerative diseases. In this article, an overview for basic care and maintenance procedures for the planarian species Schmidtea mediterranea is provided. In addition, protocols for two commonly used techniques are provided: RNAi silencing of specific genes and the use of whole-mount in situ hybridization to sensitively visualize gene expression.


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