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dc.contributor.advisorShahidullah, Mohammad
dc.contributor.authorEspiritu, Brian Gerard
dc.creatorEspiritu, Brian Gerarden_US
dc.date.accessioned2013-08-07T22:33:45Z
dc.date.available2013-08-07T22:33:45Z
dc.date.issued2013
dc.identifier.urihttp://hdl.handle.net/10150/297539
dc.description.abstractThe ciliary epithelium (CE), the tissue responsible for aqueous humor (AH) production, is the outer layer of the ciliary process and is composed of the pigmented epithelium (PE) and the nonpigmented epithelium (NPE). Previous studies have shown that inhibition of Na,K-ATPase in the CE by ouabain causes a 62% decrease in AH formation and that there is more Na,K-ATPase in the NPE than in the PE. In addition, cinnamaldehyde (CA) has been shown to inhibit Na,KATPase and other ATPases in different tissues and organisms, such as rat hepatocyte and certain bacteria. The purpose of this study was to determine if trans-CA inhibits Na,K-ATPase in rabbit NPE (rNPE). Na,K-ATPase activity was measured using a colorimetric method quantifying the amount of ATP hydrolyzed. Rb⁺ which is transported by Na,K-ATPase in a way similar to K⁺, was measured through atomic absorption spectrophotometry. The present study indicates that trans-CA has a linear concentration-dependent inhibitory effect on Na,K-ATPase and non-Na,K-ATPase activity in rNPE at concentrations of 25 and 50 μM. Trans-CA may be used as a compound to inhibit Na,K-ATPase in the CE in order to decrease aqueous humor production and intraocular pressure.
dc.language.isoenen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.titleThe Effect of Trans-Cinnamaldehyde on Na,K-ATPase Activity in Rabbit Nonpigmented Ciliary Epitheliumen_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplinePhysiologyen_US
thesis.degree.nameB.S.H.S.en_US
refterms.dateFOA2018-06-04T13:33:58Z
html.description.abstractThe ciliary epithelium (CE), the tissue responsible for aqueous humor (AH) production, is the outer layer of the ciliary process and is composed of the pigmented epithelium (PE) and the nonpigmented epithelium (NPE). Previous studies have shown that inhibition of Na,K-ATPase in the CE by ouabain causes a 62% decrease in AH formation and that there is more Na,K-ATPase in the NPE than in the PE. In addition, cinnamaldehyde (CA) has been shown to inhibit Na,KATPase and other ATPases in different tissues and organisms, such as rat hepatocyte and certain bacteria. The purpose of this study was to determine if trans-CA inhibits Na,K-ATPase in rabbit NPE (rNPE). Na,K-ATPase activity was measured using a colorimetric method quantifying the amount of ATP hydrolyzed. Rb⁺ which is transported by Na,K-ATPase in a way similar to K⁺, was measured through atomic absorption spectrophotometry. The present study indicates that trans-CA has a linear concentration-dependent inhibitory effect on Na,K-ATPase and non-Na,K-ATPase activity in rNPE at concentrations of 25 and 50 μM. Trans-CA may be used as a compound to inhibit Na,K-ATPase in the CE in order to decrease aqueous humor production and intraocular pressure.


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