Visualizing an RB-E2F Cellular Switch that Controls Cell Proliferation
PublisherThe University of Arizona.
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AbstractMammalian cell proliferation is regulated by an Rb-E2F gene network. The input node of this network, Cyclin D, receives graded growth signals; the output node, E2F, generates an all-or-none response. That is, the Rb-E2F gene network functions as a cellular switch, converting analog growth signals into digital E2F activities. The On or Off of this Rb-E2F switch determines the On or Off of cell proliferation. To help better understand the analog/digital conversion mechanism, we constructed a reporter cell line to visualize the dynamic expression of Cyclin D and E2F genes by red and green fluorescence in individual cells, respectively.
Degree ProgramHonors College