PublisherThe University of Arizona.
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AbstractSgrAI restriction enzymes bind to DNA at multiple sites and move to a central location on the molecule, forming a cluster. We qualitatively confirmed this behavior using a tethered particle motion assay and a DNA stretching assay using an optical trap. Quantitative analysis proved impossible due to the length of our DNA molecules and the large number of SgrAI binding sites. We recommend using a plasmid with a small number of binding sites for future experiments.
Degree ProgramHonors College