SgrAI Clustering on DNA
Publisher
The University of Arizona.Rights
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.Abstract
SgrAI restriction enzymes bind to DNA at multiple sites and move to a central location on the molecule, forming a cluster. We qualitatively confirmed this behavior using a tethered particle motion assay and a DNA stretching assay using an optical trap. Quantitative analysis proved impossible due to the length of our DNA molecules and the large number of SgrAI binding sites. We recommend using a plasmid with a small number of binding sites for future experiments.Type
textElectronic Thesis
Degree Name
B.S.Degree Level
bachelorsDegree Program
Honors CollegePhysics