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dc.contributor.advisorVisscher, Koen
dc.contributor.authorHendel, Edward Stephen*
dc.creatorHendel, Edward Stephenen_US
dc.date.accessioned2013-08-08T18:04:59Z
dc.date.available2013-08-08T18:04:59Z
dc.date.issued2013
dc.identifier.urihttp://hdl.handle.net/10150/297647
dc.description.abstractSgrAI restriction enzymes bind to DNA at multiple sites and move to a central location on the molecule, forming a cluster. We qualitatively confirmed this behavior using a tethered particle motion assay and a DNA stretching assay using an optical trap. Quantitative analysis proved impossible due to the length of our DNA molecules and the large number of SgrAI binding sites. We recommend using a plasmid with a small number of binding sites for future experiments.
dc.language.isoenen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.titleSgrAI Clustering on DNAen_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplinePhysicsen_US
thesis.degree.nameB.S.en_US
refterms.dateFOA2018-08-14T01:13:35Z
html.description.abstractSgrAI restriction enzymes bind to DNA at multiple sites and move to a central location on the molecule, forming a cluster. We qualitatively confirmed this behavior using a tethered particle motion assay and a DNA stretching assay using an optical trap. Quantitative analysis proved impossible due to the length of our DNA molecules and the large number of SgrAI binding sites. We recommend using a plasmid with a small number of binding sites for future experiments.


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