The Regulation of Bile Acids on NHE8 Gene Expression

Abstract

Sodium is an essential electrolyte necessary to keep multiple systems in the human body functional. Knowledge of sodium-proton exchanger will give rise to multiple clinical applications to diseases that affect our current population, which is why I personally am interested in conducting research in this field. In order to understand GW 4064 effect on NHE8 gene expression, protein and RNA expression were investigated via treatment at different concentrations and different time intervals through Western blotting and Real-Time PCR Analysis. To find the mechanism behind GW 4064 effects on NHE 8 expression, the PGL3 FXR promoter was utilized to see if GW 4064 inhibitory mechanism was through transcription or translation. Expression, stimulation, and inhibition were determined via numerical calculations. From the resulting data, we can conclude that GW 4064 inhibition of NHE8 protein and RNA expression occurs via transcription.