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Alternating Current Electrokinetic Manipulation and Concentration of Free Circulating DNA from Blood Samples

dc.contributor.advisorWong, Pak K.en_US
dc.contributor.authorLamanda, Ariana Corinne
dc.creatorLamanda, Ariana Corinneen_US
dc.date.accessioned2014-10-16T19:32:04Z
dc.date.available2014-10-16T19:32:04Z
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10150/332828
dc.description.abstractMolecular analysis of free circulating (fc)DNA has the potential to change the face of medicine, specifically in cancer diagnostics and in monitoring the efficacy of cancer treatments. In this study, a microfluidic device using AC electrokinetics is developed for rapid concentration and detection of fcDNA from blood. The device concentrates fcDNA using a combination of AC electrothermal flow and dielectrophoresis. The electrothermal fluid motion drives fcDNA towards the center of the electrode where dielectrophoretic trapping occurs. Once fcDNA is collected at the center, the concentration in the sample can be determined by fluorescent analysis using an intercalating dye binding to the double-stranded DNA. Effects of operating parameters are investigated to optimize the device's design. The electrokinetic device isolates high molecular weight DNA and can distinguish from low molecular weight DNA. Quantitative detection of fcDNA in physiologically relevant concentrations is demonstrated toward rapid diagnostics of cancer and monitoring of treatment efficacy.
dc.language.isoen_USen
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.subjectcancer diagnosticsen_US
dc.subjectcancer prognosticsen_US
dc.subjectdielectrophoresisen_US
dc.subjectfree circulating DNAen_US
dc.subjectmicrofluidic deviceen_US
dc.subjectBiomedical Engineeringen_US
dc.subjectAC electrokineticsen_US
dc.titleAlternating Current Electrokinetic Manipulation and Concentration of Free Circulating DNA from Blood Samplesen_US
dc.typetexten
dc.typeElectronic Thesisen
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.levelmastersen_US
dc.contributor.committeememberWong, Pak K.en_US
dc.contributor.committeememberVande Geest, Jonathan P.en_US
dc.contributor.committeememberWu, Xiaoyien_US
dc.contributor.committeememberUtzinger, Ursen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.disciplineBiomedical Engineeringen_US
thesis.degree.nameM.S.en_US
refterms.dateFOA2018-06-14T23:52:39Z
html.description.abstractMolecular analysis of free circulating (fc)DNA has the potential to change the face of medicine, specifically in cancer diagnostics and in monitoring the efficacy of cancer treatments. In this study, a microfluidic device using AC electrokinetics is developed for rapid concentration and detection of fcDNA from blood. The device concentrates fcDNA using a combination of AC electrothermal flow and dielectrophoresis. The electrothermal fluid motion drives fcDNA towards the center of the electrode where dielectrophoretic trapping occurs. Once fcDNA is collected at the center, the concentration in the sample can be determined by fluorescent analysis using an intercalating dye binding to the double-stranded DNA. Effects of operating parameters are investigated to optimize the device's design. The electrokinetic device isolates high molecular weight DNA and can distinguish from low molecular weight DNA. Quantitative detection of fcDNA in physiologically relevant concentrations is demonstrated toward rapid diagnostics of cancer and monitoring of treatment efficacy.


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