Effects of Toxaphene® on the abdominal ventral nerve cord of two cockroach species, Leucophaea maderae (F.) and Periplaneta americana (L.)
AuthorDary, Curtis Carl
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PublisherThe University of Arizona.
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AbstractEffects of Toxaphene®, a polychlorinated terpene insecticide, were studied by a multidisciplinary approach on two cockroach species, Leucophaea maderae (F.) and Periplaneta americana (L.). Toxaphene was found to be slightly toxic to L. maderae and moderately toxic to P. americana. Uptake of ³⁶C1-toxaphene in tissues of L. maderae was studied from 2 to 120 hours. Greater accumulation of ³⁶C1-toxaphene on a ug/gm basis was found associated with the abdominal ventral nerve cord (VNC) of L. maderae than with haemolymph, fat body, and alimentary canal. ³⁶C1-toxaphene uptake was noted in L. maderae VNC in vitro at levels which were independent of incubation dose concentration. Substantial amounts of ³⁶C1-toxaphene remained associated with VNC after rinsing. Radiolabeled material found in haemolymph of asymptomatic L. maderae appeared to be qualitatively different from toxaphene standards and also that found in symptomatic insect haemolymph based on gas chromatography. Concentration levels responsible for in vivo toxicity appeared to correlate with electrophysiological symptomology in VNC of P. americana. Intense forms of activity were observed in isolated nerve preparations and in VNC removed rom symptomatic insects treated with 1/10⁵ and 1/10¹ M toxaphene. Latent periods between introductioon of toxaphene and onset of intense activity appeared to vary as a function of concentration.