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    • Desert Plants, Volume 17, Number 2 (December 2001)
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    Tissue Culture and Cloning of Carnegiea gigantea, Cactaceae

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    Author
    Baker, William P.
    Hanks, Tyrone Harvard
    Marin, Louis Eduardo
    Affiliation
    Biomedical Sciences, Midwestern University
    Life Sciences Department, Mesa Community College
    Issue Date
    2015-05-20
    
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    Show full item record
    Publisher
    University of Arizona (Tucson, AZ)
    Journal
    Desert Plants
    Rights
    Copyright © Arizona Board of Regents. The University of Arizona.
    Collection Information
    Desert Plants is published by The University of Arizona for the Boyce Thompson Southwestern Arboretum. For more information about this unique botanical journal, please email the College of Agriculture and Life Sciences Publications Office at pubs@cals.arizona.edu.
    Abstract
    Cloning has become an established method of supplying valuable timber trees and other plants for commercial purposes. Cloning of these plants allows multiple copies to be produced from superior phenotypes. In this study, in vitro clones were produced from phenotypically selected, commercially available saguaro (Carnegiea gigantea). The clones were produced from tissue plugs obtained from surface sterilized saguaro. The plugs were transferred using standard aseptic technique to culture dishes containing solid Callus Initiation Medium (Gamborg's B-5 medium supplemented with 10 mg/l auxin and 8 g/l agar). The cultures were incubated under continuous cool fluorescent lights at 24 C until callus formation was observed. Healthy callus were transferred to solid Development Medium (Gamborg's B-5 medium supplemented with 10 mg/l auxin, 10.0 mg Kinetin, and 8 g/l agar) and further incubated. Resulting clones were prepared for in vivo conditions by transfer to sterile potting soil and successfully outplanted to the green house. Such clones may supply scarce C. gigantea for future research. The use of single genotypes for ecological applications should be avoided since they lack natural population variability.
    Type
    Article
    ISSN
    0734-3434
    Collections
    Desert Plants, Volume 17, Number 2 (December 2001)

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