Is Yer087w Protein in Complex with Lip3, Functioning in Lipoylation in Yeast Mitochondria?
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PublisherThe University of Arizona.
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AbstractIn yeast mitochondria, the enzyme Lip3 is involved in the transfer of octanoic acid from Gcv3, a subunit of GCV, to other targets. However, it is unknown whether Lip3 itself has acyl transferase activity, or if it serves to present the fatty acid as well as the substrate to a transferase. When Lip3 is purified by tandem tag chromatography, the final eluate contains Lip3 as well as an additional co-purifying protein. It was hypothesized that this protein may be the transferase that Lip3 associates with when transferring octanoic acid. Furthermore, based on the size and predicted properties of this co-purifying protein, it was hypothesized that Yer087w may be the transferase that co-purifies with Lip3. However, when the YER087w gene was deleted in a yeast strain where Lip3 was tagged with Protein C peptide and His-6 (notated as Lip3- CH), the co-purifying protein was still present in the final eluate, indicating that Yer087w is not that protein. This was confirmed by mass spectrometry, which determined the co-purifying protein to be Heat Shock Protein 60.
Degree ProgramHonors College