The Implications of Knocking Out Two Major Salivary Androgen-Binding Protein Genes for Mouse Viability, Fecundity and the Expression of Other ABP Genes
AuthorChung, Amanda Grace
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PublisherThe University of Arizona.
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AbstractThe Androgen-binding protein (Abp) genes in the house mouse genome have undergone extensive duplications that produced 64 Abp paralogs. Distinct sets of Abp genes are expressed in lacrimal and salivary glands. This study examines the changes in a mouse line lacking two major salivary Abp genes, Abpa27 and Abpbg27 (a27/bg27 knockout line). We observed no marked changes in lifespan or fecundity between the three genotypes. We paired traditional PCR with quantitative PCR to measure changes in Abp paralog expression in both submandibular and lacrimal glands. We observed a decrease in expression of a27 and bg27 transcripts in the salivary glands of +/- mice and a complete absence of a27 and bg27 in the salivary glands of -/- mice. We did not detect any changes in expression of any other Abp paralogs in salivary glands. On the other hand, we saw variance in Abp expression between the three genotypes in the lacrimal glands including an increase in bg10 and bg20 expression in female -/- mice, and a3 in male -/- mice. This study will be the first of many documenting the changes that occur in a knockout mouse line.
Degree ProgramHonors College