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dc.contributor.advisorKacira, Muraten
dc.contributor.authorJia, Fei
dc.creatorJia, Feien
dc.date.accessioned2015-10-01T22:24:29Zen
dc.date.available2015-10-01T22:24:29Zen
dc.date.issued2015en
dc.identifier.urihttp://hdl.handle.net/10150/579045en
dc.description.abstractA multi-wavelength laser diode based optical sensor was designed, developed and evaluated for monitoring and control microalgae growth in real-time. The sensor measures optical density of microalgae suspension at three wavelengths: 650 nm, 685 nm and 780 nm, which are commonly used for estimating microalgae biomass concentration and chlorophyll content. The sensor showed capability of measuring cell concentration up to 1.05 g L⁻¹ without sample dilution or preparation. The performance of the sensor was evaluated using both indoor photobioreactors and outdoor paddle wheel reactors. It was shown that the sensor was capable of monitoring the dynamics of the microalgae culture in real-time with high accuracy and durability. Specific growth rate (μ) and ratios of optical densities (OD ratios) at different wavelengths were calculated and were used as good indicators of the health of microalgae culture. A series of experiments was conducted to evaluate the sensor's capability of detecting environmental disturbances in microalgae systems, for instance, induced by dust or Vampirovibrio chlorellavorus, a bacteria found to cause crash of microalgae culture. Optical densities measured from the sensor were insensitive to the amount of dust that consisted of 59.7% of dry weight of microalgae in the system. However, the sensor was able to detect multiple events of introduction of dust timely by μ and OD ratios. The sensor was also capable of detecting subtle changes of culture in color that leads to a total crash of the culture before it can be differentiated by naked eye. The sensor was further integrated into an existing outdoor raceway to demonstrate the sensor's potential of being a core component to control microalgae production system. A real-time monitoring and control program along with a graphical user interface (GUI) was developed for a central control station aiming at improving resource use efficiency for biomass production.
dc.language.isoen_USen
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.subjectMicroalgaeen
dc.subjectMulti-wavelengthen
dc.subjectOptical densityen
dc.subjectReal-time monitoring and controlen
dc.subjectVampirovibrio chlorellavorusen
dc.subjectAgricultural & Biosystems Engineeringen
dc.subjectEarly detectionen
dc.titleMultivariable And Sensor Feedback Based Real-Time Monitoring And Control Of Microalgae Production Systemen_US
dc.typetexten
dc.typeElectronic Dissertationen
thesis.degree.grantorUniversity of Arizonaen
thesis.degree.leveldoctoralen
dc.contributor.committeememberKacira, Muraten
dc.contributor.committeememberAn, Linglingen
dc.contributor.committeememberBrown, Judith K.en
dc.contributor.committeememberOgden, Kimberly L.en
dc.description.releaseRelease 12-Aug-2016en
thesis.degree.disciplineGraduate Collegeen
thesis.degree.disciplineAgricultural & Biosystems Engineeringen
thesis.degree.namePh.D.en
refterms.dateFOA2016-08-12T00:00:00Z
html.description.abstractA multi-wavelength laser diode based optical sensor was designed, developed and evaluated for monitoring and control microalgae growth in real-time. The sensor measures optical density of microalgae suspension at three wavelengths: 650 nm, 685 nm and 780 nm, which are commonly used for estimating microalgae biomass concentration and chlorophyll content. The sensor showed capability of measuring cell concentration up to 1.05 g L⁻¹ without sample dilution or preparation. The performance of the sensor was evaluated using both indoor photobioreactors and outdoor paddle wheel reactors. It was shown that the sensor was capable of monitoring the dynamics of the microalgae culture in real-time with high accuracy and durability. Specific growth rate (μ) and ratios of optical densities (OD ratios) at different wavelengths were calculated and were used as good indicators of the health of microalgae culture. A series of experiments was conducted to evaluate the sensor's capability of detecting environmental disturbances in microalgae systems, for instance, induced by dust or Vampirovibrio chlorellavorus, a bacteria found to cause crash of microalgae culture. Optical densities measured from the sensor were insensitive to the amount of dust that consisted of 59.7% of dry weight of microalgae in the system. However, the sensor was able to detect multiple events of introduction of dust timely by μ and OD ratios. The sensor was also capable of detecting subtle changes of culture in color that leads to a total crash of the culture before it can be differentiated by naked eye. The sensor was further integrated into an existing outdoor raceway to demonstrate the sensor's potential of being a core component to control microalgae production system. A real-time monitoring and control program along with a graphical user interface (GUI) was developed for a central control station aiming at improving resource use efficiency for biomass production.


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