ncRNA Targeting CSF-1 and CSF1R Expression in Breast and Ovarian Cancer Cells

Abstract

Various sense ncRNA transcripts targeting nucleolin and vigilin, and shRNA transcripts targeting CSF-1 and c-fms mRNAs were introduced into Hey ovarian cancer and BT20 breast cancer cell lines to measure their influence on CSF1R and CSF1 protein expression. In BT20 cells transcripts c-fms-C-rich 104-S, c-fms-(CS-rCS), and c-fms-Py-S were found to downregulate CSF1R expression. In BT20 cells transcripts CSF-1-Exon-10-356-S and CSF-1 (CS-rCS) were found to have a downregulatory effect on CSF1 expression while transcript CSF-1-5'UTR-420-S was found to upregulate CSF1 expression. In Hey cells transcript CSF-1-3'UTR-AS-Pyrimidine was found to downregulate CSF1 expression while transcript CSF-1mRNA-Variant-4-5'UTR was found to have upregulate CSF1 expression. Also in Hey cells, both transcripts c-fms C-rich-104-S and c-fms-Py-S were found to upregulate CSF1R expression. These results indicate that ncRNAs are able to have a regulatory effect on CSF1 and CSF1R expression but also suggest that the mechanism of regulation is complex. Furthermore, the complex mechanism makes it difficult to predict if the transcript will have its intended effect. We concluded that differences in baseline RNA binding protein concentrations and other regulatory mechanisms, such as microRNAs, account for the conflicting results between the BT20 and Hey cell lines when comparing the same inserted transcript.