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    ncRNA Targeting CSF-1 and CSF1R Expression in Breast and Ovarian Cancer Cells

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    Author
    Patel, Arpan Ajit
    Issue Date
    2015
    Advisor
    Chambers, Setsuko
    Woo, Ho-Hyung
    
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    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    Various sense ncRNA transcripts targeting nucleolin and vigilin, and shRNA transcripts targeting CSF-1 and c-fms mRNAs were introduced into Hey ovarian cancer and BT20 breast cancer cell lines to measure their influence on CSF1R and CSF1 protein expression. In BT20 cells transcripts c-fms-C-rich 104-S, c-fms-(CS-rCS), and c-fms-Py-S were found to downregulate CSF1R expression. In BT20 cells transcripts CSF-1-Exon-10-356-S and CSF-1 (CS-rCS) were found to have a downregulatory effect on CSF1 expression while transcript CSF-1-5'UTR-420-S was found to upregulate CSF1 expression. In Hey cells transcript CSF-1-3'UTR-AS-Pyrimidine was found to downregulate CSF1 expression while transcript CSF-1mRNA-Variant-4-5'UTR was found to have upregulate CSF1 expression. Also in Hey cells, both transcripts c-fms C-rich-104-S and c-fms-Py-S were found to upregulate CSF1R expression. These results indicate that ncRNAs are able to have a regulatory effect on CSF1 and CSF1R expression but also suggest that the mechanism of regulation is complex. Furthermore, the complex mechanism makes it difficult to predict if the transcript will have its intended effect. We concluded that differences in baseline RNA binding protein concentrations and other regulatory mechanisms, such as microRNAs, account for the conflicting results between the BT20 and Hey cell lines when comparing the same inserted transcript.
    Type
    text
    Electronic Thesis
    Degree Name
    B.S.
    Degree Level
    bachelors
    Degree Program
    Honors College
    Molecular and Cellular Biology
    Degree Grantor
    University of Arizona
    Collections
    Honors Theses

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