• Canine Irradiated Spherule Vaccine Trial

      Reed, Raymond E.; The University of Arizona (The University of Arizona, 2016-09-27)
    • Canine Irradiated Spherule Vaccine Trial

      Reed, Raymond E.; The University of Arizona (The University of Arizona, 2016-09-27)
    • Canine Irradiated Spherule Vaccine Trial

      Reed, Raymond E.; The University of Arizona (The University of Arizona, 2016-09-26)
    • Canine Irradiated Spherule Vaccine Trial

      Reed, Raymond E.; The University of Arizona (The University of Arizona, 2016-09-27)
    • Canine Irradiated Spherule Vaccine Trial

      Reed, Raymond E.; The University of Arizona (The University of Arizona, 2016-09-27)
    • No Effect of Acute Eccentric Resistance Exercise on Immune Responses to Influenza Vaccination in Older Adults: A Randomized Control Trial

      Elzayat, M.T.; Markofski, M.M.; Simpson, R.J.; Laughlin, M.; LaVoy, E.C.; Department of Nutritional Sciences, College of Agriculture and Life Sciences, University of Arizona (Frontiers Media S.A., 2021)
      Introduction: Older adults are at elevated risk for morbidity and mortality caused by influenza. Vaccination is the primary means of prophylaxis, but protection is often compromised in older adults. As resistance exercise mobilizes immune cells into muscle, it may enhance vaccination response. Purpose: Compare antibody and cell mediated immune responses to influenza vaccination in older adults who performed eccentric resistance exercise immediately prior to vaccination to those who did not exercise. Methods: Twenty nine resistance training-naive older adults (20 women, 73.9 ± 5.3 years) were randomized to 1 of 3 groups: vaccination in the same arm that exercised (Ex-S), vaccination in the opposite arm that exercised (Ex-Op), and seated rest (No-Ex). Exercise consisted of 10 sets of 5 eccentric unilateral repetitions at 80% of the pre-determined concentric one repetition maximum. Lateral raises were alternated with bicep curls. No-Ex sat quietly for 25 min. Following exercise or rest, all received the 2018 quadrivalent influenza vaccine (Seqirus Afluria) in the non-dominant deltoid. Antibody titers against each influenza vaccine strain were determined by hemagglutinin inhibition assays at baseline, 6-, and 24-weeks post-vaccination. Influenza-specific T cells were quantified after stimulation with the vaccine by intracellular cytokine staining. Results: No significant group x time effects were found in antibody responses to any strain (interaction for A/H1N1: p = 0.682; A/H3N2: p = 0.644; B/Colorado/06/2017: p = 0.262; B/Phuket/3073/2013: p = 0.851). Groups did not differ in fold-increase of antibody titers 6- and 24-weeks post-vaccination. Influenza-specific T-cells did not differ between groups at any time (comparison at baseline: p = 0.985; 6-weeks: p = 0.889; 24 weeks: p = 0.857). One subject (Ex-S) reported flu-like symptoms 18 weeks post-vaccination. Conclusion: Acute arm eccentric exercise did not influence antibody titers or cell mediated immune responses to the influenza vaccine delivered post-exercise in older adults. More strenuous exercise may be required for exercise to act as an adjuvant. ClinicalTrials.gov Identifier: NCT03736759. © Copyright © 2021 Elzayat, Markofski, Simpson, Laughlin and LaVoy.
    • Substrate-source flexibility of an exponential-fed perfusion process to produce plasmid DNA for use as leishmaniasis vaccine

      García-Rendón, Aurora; García-Rendón, Angelica; Guzmán, Roberto; Tejeda-Mansir, Armando; Univ Arizona, Dept Chem & Environm Engn (TAYLOR & FRANCIS LTD, 2019-01-10)
      The use of plasmid DNA (pDNA) for human vaccines is a novel approach against leishmaniasis, a neglected tropical disease with severe clinical manifestations. The development of feasible bioprocesses to obtain such vaccines is a public-health priority. The aim of this work was to investigate the substrate-source flexibility of an exponential-fed perfusion (EFP) system to produce the plasmid pVAX1-NH36 for use as a leishmaniasis vaccine. Batch and EFP cultures were conducted using Escherichia coli DH5 alpha as a host and glucose or glycerol as a carbon source. The culture kinetics of the cell, substrate and plasmid concentrations were measured. Mathematical kinetics models were fitted to experimental data and used to describe the system comportment (r(2) > 0.95). Plasmid productivities of 13.3 mg/(L h) using glucose and 19.4 mg/(L h) using glycerol were obtained. These levels represent a 1-3-fold increase in performance index compared with previously reported cultures using E. coli DH5 alpha. The novel aspect of this work is the demonstration of the flexibility of EFP cultures for production of pDNA vaccines. Our data suggest that E. coli engineering to increase pDNA production using glucose can be circumvented with an EFP culture, reducing the host strain development costs. In addition, the greater productivity of EFP cultures entails a reduction in manufacturing costs.