Now showing items 10169-10188 of 12955

    • S 2 Rydberg spectrum of the boron atom

      Hornyák, I.; Nasiri, S.; Bubin, S.; Adamowicz, L.; Department of Physics, University of Arizona (American Physical Society, 2021)
      Benchmark variational calculations of the lowest ten Rydberg S2 states of two stable isotopes of the boron atom (B10 and B11) are reported. The nonrelativistic wave functions of this five-electron system are expanded in terms of 16 000 all-particle explicitly correlated Gaussians (ECGs). The ECG nonlinear exponential parameters are extensively optimized using a procedure that employs the analytic gradient of the energy with respect to these parameters. A finite nuclear mass value is used in the calculations and the motion of the nucleus is explicitly represented in the nonrelativistic Hamiltonian. The leading relativistic corrections to the energy levels are computed in the framework of the perturbation theory. The lowest-order quantum electrodynamics corrections are also estimated. The results obtained for the energy levels enable determination of interstate transition frequencies with accuracy that approaches the available experimental spectroscopic data. © 2021 American Physical Society.
    • (S)-lacosamide inhibition of CRMP2 phosphorylation reduces postoperative and neuropathic pain behaviors through distinct classes of sensory neurons identified by constellation pharmacology.

      Moutal, Aubin; Chew, Lindsey A; Yang, Xiaofang; Wang, Yue; Yeon, Seul Ki; Telemi, Edwin; Meroueh, Seeneen; Park, Ki Duk; Shrinivasan, Raghuraman; Gilbraith, Kerry B; et al. (LIPPINCOTT WILLIAMS & WILKINS, 2016-07)
      Chronic pain affects the life of millions of people. Current treatments have deleterious side effects. We have advanced a strategy for targeting protein interactions which regulate the N-type voltage-gated calcium (CaV2.2) channel as an alternative to direct channel block. Peptides uncoupling CaV2.2 interactions with the axonal collapsin response mediator protein 2 (CRMP2) were antinociceptive without effects on memory, depression, and reward/addiction. A search for small molecules that could recapitulate uncoupling of the CaV2.2-CRMP2 interaction identified (S)-lacosamide [(S)-LCM], the inactive enantiomer of the Food and Drug Administration-approved antiepileptic drug (R)-lacosamide [(R)-LCM, Vimpat]. We show that (S)-LCM, but not (R)-LCM, inhibits CRMP2 phosphorylation by cyclin dependent kinase 5, a step necessary for driving CaV2.2 activity, in sensory neurons. (S)-lacosamide inhibited depolarization-induced Ca influx with a low micromolar IC50. Voltage-clamp electrophysiology experiments demonstrated a commensurate reduction in Ca currents in sensory neurons after an acute application of (S)-LCM. Using constellation pharmacology, a recently described high content phenotypic screening platform for functional fingerprinting of neurons that uses subtype-selective pharmacological agents to elucidate cell-specific combinations (constellations) of key signaling proteins that define specific cell types, we investigated if (S)-LCM preferentially acts on certain types of neurons. (S)-lacosamide decreased the dorsal root ganglion neurons responding to mustard oil, and increased the number of cells responding to menthol. Finally, (S)-LCM reversed thermal hypersensitivity and mechanical allodynia in a model of postoperative pain, and 2 models of neuropathic pain. Thus, using (S)-LCM to inhibit CRMP2 phosphorylation is a novel and efficient strategy to treat pain, which works by targeting specific sensory neuron populations.
    • Saccade-related neural communication in the human medial temporal lobe is modulated by the social relevance of stimuli

      Staudigl, T.; Minxha, J.; Mamelak, A.N.; Gothard, K.M.; Rutishauser, U.; Department of Physiology, College of Medicine, University of Arizona (American Association for the Advancement of Science, 2022)
      Humans predominantly explore their environment by moving their eyes. To optimally communicate and process visual information, neural activity needs to be coordinated with the execution of eye movements. We investigated the coordination between visual exploration and interareal neural communication by analyzing local field potentials and single neuron activity in patients with epilepsy. We demonstrated that during the free viewing of images, neural communication between the human amygdala and hippocampus is coordinated with the execution of eye movements. The strength and direction of neural communication and hippocampal saccade-related phase alignment were strongest for fixations that landed on human faces. Our results argue that the state of the human medial temporal lobe network is selectively coordinated with motor behavior. Interareal neural communication was facilitated for social stimuli as indexed by the category of the attended information. © 2022 The Authors.
    • Safety and Efficacy of Daptomycin in Neonates with Coagulase-Negative Staphylococci: Case Series Analysis

      Mohzari, Yahya; Aljobair, Fahad; Alrashed, Ahmed; Asdaq, Syed Mohammed Basheeruddin; Alshuraim, Renad Abdullah; Asfour, Suzan Suhail; Al-Mouqdad, Mountasser Mohammad; Bamogaddam, Reem F.; Al-Anazi, Deemah; Zeilinger, Catherine E.; et al. (MDPI AG, 2021-02-07)
      There has been an increase in the prevalence of gram-positive bacteremia in neonates in the last two decades. However, as a consequence of better care, there has been an increase in the survival of premature neonates. Coagulase-negative staphylococci (CoNS) is the most prevalent bacteria, responsible for up to 60% of late-onset sepsis (LOS). Daptomycin, a lipopeptide antimicrobial agent, is active against CoNS. This was an observational, retrospective case series study carried out in the Pediatric Hospital of King Saud Medical City, Riyadh, Saudi Arabia. The medical records of 21 neonates, aged 0–28 days, who were treated in Neonatal Intensive Care Unit (NICU) with intravenous daptomycin as monotherapy or combination therapy for at least 4 days for proven gram-positive infection between June 2019 to July 2020, were included. The median gestational and chronological age were 27 weeks and 5 days, respectively. The most frequent diagnosis in neonates was infective endocarditis (42.9%). Of the 21 patients who received daptomycin therapy, 13 (62%) recovered and 8 died. The clinical cure rate was higher in Staphylococcus hominis (100%) and in patients who received 6 mg/kg/dose twice daily (62.5%). The mean of aspartate aminotransferase significantly elevated after starting daptomycin (p = 0.048). However, no muscular or neurological toxicity of daptomycin was documented in any of the cases. Overall, daptomycin was well tolerated, even with long-term treatment. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
    • Safety and efficacy of ex vivo expanded CD34 stem cells in murine and primate models

      Zhang, Yu; Shen, Bin; Guan, Xin; Qin, Meng; Ren, Zhihua; Ma, Yupo; Dai, Wei; Ding, Xinxin; Jiang, Yongping; Univ Arizona, Coll Pharm, Dept Pharmacol & Toxicol (BMC, 2019-06-13)
      Background: Hematopoietic stem cell (HSC) transplantation has been widely applied to the treatment of malignant blood diseases. However, limited number of functional HSCs hinders successful transplantation. The purpose of our current study is to develop a new and cost-efficient medium formulation that could greatly enhance the expansion of HSCs while retaining their long-term repopulation and hematopoietic properties for effective clinical transplantation. Methods: Enriched human CD34(+) cells and mobilized nonhuman primate peripheral blood CD34(+) cells were expanded with a new, cost-efficient expansion medium formulation, named hematopoietic expansion medium (HEM), consisting of various cytokines and nutritional supplements. The long-term repopulation potential and hematologic-lineage differentiation ability of expanded human cells were studied in the non-obese diabetic/severe combined immunodeficiency mouse model. Furthermore, the efficacy and safety studies were performed by autologous transplantation of expanded primate cells in the nonhuman primate model. Results: HEM could effectively expand human CD34(+) cells by up to 129 fold within 9 days. Expanded HSCs retained long-term repopulation potential and hematologic-lineage differentiation ability, as indicated by (1) maintenance (over unexpanded HSCs) of immunophenotypes of CD38(-)CD90(+)CD45RA(-)CD49f(+) in CD34(+) cells after expansion; (2) significant presence of multiple human hematopoietic lineages in mouse peripheral blood and bone marrow following primary transplantation; (3) enrichment (over unexpanded HSCs) in SCID-repopulating cell frequency measured by limiting dilution analysis; and (4) preservation of both myeloid and lymphoid potential among human leukocytes from mouse bone marrow in week 24 after primary transplantation or secondary transplantation. Moreover, the results of autologous transplantation in nonhuman primates demonstrated that HEM-expanded CD34(+) cells could enhance hematological recovery after myelo-suppression. All primates transplanted with the expanded autologous CD34(+) cells survived for over 18 months without any noticeable abnormalities. Conclusions: Together, these findings demonstrate promising potential for the utility of HEM to improve expansion of HSCs for clinical application.
    • Safety and efficacy of rivaroxaban in pediatric cerebral venous thrombosis (Einstein-Jr CVT)

      Connor, P.; van Kammen, M.S.; Lensing, A.W.A.; Chalmers, E.; Kállay, K.; Hege, K.; Simioni, P.; Biss, T.; Bajolle, F.; Bonnet, D.; et al. (American Society of Hematology, 2020)
      Anticoagulant treatment of pediatric cerebral venous thrombosis has not been evaluated in randomized trials. We evaluated the safety and efficacy of rivaroxaban and standard anticoagulants in the predefined subgroup of children with cerebral venous thrombosis (CVT) who participated in the EINSTEIN-Jr trial. Children with CVT were randomized (2:1), after initial heparinization, to treatment with rivaroxaban or standard anticoagulants (continued on heparin or switched to vitamin K antagonist). The main treatment period was 3 months. The primary efficacy outcome, symptomatic recurrent venous thromboembolism (VTE), and principal safety outcome, major or clinically relevant nonmajor bleeding,were centrally evaluated by blinded investigators. Sinus recanalization on repeat brain imaging was a secondary outcome. Statistical analyses were exploratory. In total, 114 children with confirmed CVT were randomized. All children completed the follow-up. None of the 73 rivaroxaban recipients and 1 (2.4%; CVT) of the 41 standard anticoagulant recipients had symptomatic, recurrent VTE after 3 months (absolute difference, 2.4%; 95% confidence interval [CI], 22.6% to 13.5%). Clinically relevant bleeding occurred in 5 (6.8%; all nonmajor and noncerebral) rivaroxaban recipients and in 1 (2.5%; major [subdural] bleeding) standard anticoagulant recipient (absolute difference, 4.4%; 95% CI, 26.7% to 13.4%). Complete or partial sinus recanalization occurred in 18 (25%) and 39 (53%) rivaroxaban recipients and in 6 (15%) and 24 (59%) standard anticoagulant recipients, respectively. In summary, in this substudy of a randomized trial with a limited sample size, children with CVT treated with rivaroxaban or standard anticoagulation had a low risk of recurrent VTE and clinically relevant bleeding. This trial was registered at as #NCT02234843. © 2020 by The American Society of Hematology
    • Safety and Efficacy of Stent-assisted Coiling in the Treatment of Unruptured Wide-necked Intracranial Aneurysms: A Single-center Experience

      Aguilar-Salinas, Pedro; Brasiliense, Leonardo B; Santos, Roberta; Cortez, Gustavo M; Gonsales, Douglas; Aghaebrahim, Amin; Sauvageau, Eric; Hanel, Ricardo A; Univ Arizona, Neurosurg (CUREUS INC, 2019-06-06)
      Introduction: Wide-necked intracranial aneurysms (IAs) are complex lesions that may require different microsurgical or endovascular strategies, and stent-assisted coiling (SAC) has emerged as a feasible alternative to treat this subset of aneurysms. Methods: The objective was to assess the rate of complications of unruptured wide-necked IAs treated with SAC. We retrospectively identified patients with unruptured wide-necked IAs treated with SAC. Medical charts, procedure reports, and imaging studies were analyzed. Results: One hundred twenty patients harboring 124 unruptured wide-necked IAs were included. Ninety-two aneurysms (74.2%) were located in the anterior circulation. The median aneurysm size was 7 mm (IQR = 5-10). The immediate complete aneurysm occlusion rate was 29% (36/124). The rate of procedural complications was 3.3 % (4/120), which included 2 intraprocedural aneurysm ruptures, 1 immediate postprocedure aneurysm rupture, and 1 vessel occlusion rescued with an open-cell stent. The median follow-up time was 21 months (IQR = 10.3-40.9). Kaplan-Meier analysis estimated a median time of complete aneurysm occlusion of 6.3 months (95%CI = 3.8-7.8). At 30-day follow-up, 80.7% of patients had a Glasgow Outcome Score (GOS) of 5 and at the latest follow-up 83.9%. Imaging follow-up was available for 102 patients. The rate of complete aneurysm occlusion was 73.5% (75/102), severe in-stent stenosis (>50%) was found in 1% (1/102), the recanalization rate was 6.6% (5/75), and the retreatment rate was 7.8% (8/102). Conclusion: SAC remains a safe and effective technique to treat wide-necked IAs, providing low rate of complications and recanalization with excellent long-term aneurysm occlusion rates.
    • Safety and tolerability of srx246, a vasopressin 1a antagonist, in irritable huntington’s disease patients—a randomized phase 2 clinical trial

      Brownstein, M.J.; Simon, N.G.; Long, J.D.; Yankey, J.; Maibach, H.T.; Cudkowicz, M.; Coffey, C.; Conwit, R.A.; Lungu, C.; Anderson, K.E.; et al. (MDPI, 2020)
      SRX246 is a vasopressin (AVP) 1a receptor antagonist that crosses the blood‐brain barrier. It reduced impulsive aggression, fear, depression and anxiety in animal models, blocked the actions of intranasal AVP on aggression/fear circuits in an experimental medicine fMRI study and demonstrated excellent safety in Phase 1 multiple‐ascending dose clinical trials. The present study was a 3‐arm, multicenter, randomized, placebo‐controlled, double‐blind, 12‐week, dose escalation study of SRX246 in early symptomatic Huntington’s disease (HD) patients with irritability. Our goal was to determine whether SRX246 was safe and well tolerated in these HD patients given its potential use for the treatment of problematic neuropsychiatric symptoms. Participants were randomized to receive placebo or to escalate to 120 mg twice daily or 160 mg twice daily doses of SRX246. Assessments included standard safety tests, the Unified Huntington’s Disease Rating Scale (UHDRS), and exploratory measures of problem behaviors. The groups had comparable demographics, features of HD and baseline irritability. Eighty‐two out of 106 subjects randomized completed the trial on their assigned dose of drug. One‐sided exact‐method confidence interval tests were used to reject the null hypothesis of inferior tolerability or safety for each dose group vs. placebo. Apathy and suicidality were not affected by SRX246. Most adverse events in the active arms were considered unlikely to be related to SRX246. The compound was safe and well tolerated in HD patients and can be moved forward as a candidate to treat irritability and aggression. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
    • Safety assessment of genetically modified crops for yield increase and resistance to both biotic and abiotic stresses and their impact on human and environment

      Askari-Khorasgani, Omid; Pessarakli, Mohammad; Univ Arizona, Sch Plant Sci (MedCrave, 2018-03-06)
      Biotechnology has now accelerated the breeding evolution of agricultural products to obtain stress-tolerant crops with improved productivity to meet the increasing global demand which is in direct correlation with demographic trends. Nutritional quality, food allergies, toxicity effects, and unwanted resistance and alternations in gene sequences are the main concerns of genetically engineered products. Despite the numerous and overall benefits of genetically engineered crops, the questions raised by unwanted and unpredictable consequences about their safety concerns that may harm human, animal, and/or the ecosystem health require a long-term and a case-by-case transparent assessments before releasing the biotech crops into the food chain.
    • The SAGA Survey. I. Satellite Galaxy Populations around Eight Milky Way Analogs

      Geha, Marla; Wechsler, Risa H.; Mao, Yao-Yuan; Tollerud, Erik J.; Weiner, Benjamin; Bernstein, Rebecca; Hoyle, Ben; Marchi, Sebastian; Marshall, Phil J.; Muñoz, Ricardo; et al. (IOP PUBLISHING LTD, 2017-09-14)
      We present the survey strategy and early results of the "Satellites Around Galactic Analogs" (SAGA) Survey. The SAGA. Survey's goal is to measure the distribution of satellite galaxies around 100 systems analogous to the Milky Way down to the luminosity of the Leo I dwarf galaxy (M-r < -12.3). We define a Milky Way analog based on K-band luminosity and local environment. Here, we present satellite luminosity functions for eight Milky-Way-analog galaxies between 20 and 40. Mpc. These systems have nearly complete spectroscopic coverage of candidate satellites within the projected host virial radius down to r(o) < 20.75 using low-redshift gri color criteria. We have discovered a total of 25 new satellite galaxies: 14. new satellite galaxies meet our formal criteria around our complete host systems, plus 11 additional satellites in either incompletely surveyed hosts or below our formal magnitude limit. Combined with 13 previously known satellites, there are a total of 27 satellites around 8 complete Milky-Way-analog hosts. We find a wide distribution in the number of satellites per host, from 1 to 9, in the luminosity range for which there are 5 Milky Way satellites. Standard abundance matching extrapolated from higher luminosities predicts less scatter between hosts and a steeper luminosity function slope than observed. We find that the majority of satellites (26 of 27) are star-forming. These early results indicate that the Milky Way has a different satellite population than typical in our sample, potentially changing the physical interpretation of measurements based only on the Milky Way's satellite galaxies.
    • Salicylic acid regulates photosynthetic electron transfer and stomatal conductance of mung bean (Vigna radiata L.) under salinity stress

      Lotfi, Ramin; Ghassemi-Golezani, Kazem; Pessarakli, Mohammad; Univ Arizona, Coll Agr & Life Sci, Sch Plant Sci (ELSEVIER, 2020-07)
      The role of salicylic acid (0, 1 and 1.5 mM) on photosynthetic electron transfer chain of mung-bean plants grown under salt stress (0, 3, 6 and 9 dS/m(2)) was studied using chlorophyll a fluorescence (ChlF) measurements. Results indicated that accumulation of K+ content decreased but, Na+ content increased with increasing salt stress. SA-treated plants had more K+ and less Na+ content compared with the non-SA treated plants. Application of SA, especially with 1 mM, increased the I-P step of the OJIP transient curve of fluorescence. Salt stress decreased g(s), CCI, F-V, F-M, S-M and PIabs in plants. However, the time taken to reach FM (TFM) increased with increasing salt stress. Application of different concentrations of SA significantly improved gs, CCI, FV, SM, and PIabs of plants. Photosynthetic efficiency of plants improved as a result of SA application via decreasing F-o and increasing F-V/F-M, F-V/F-O, S-M/T-FM and Area under both saline and non-saline conditions. Accumulation of Na+ had negative, but K+ had a positive correlation with gs, CCI and most of the chlorophyll a fluorescence parameters (except, Fo and TFM). A positive correlation was found between g(s) and CCI with PIabs. This research indicated that low gs under saline condition seems to cause losses in PSII efficiency, but the application of SA with 1 mM concentration is the best treatment for the alleviation of salt stress injuries on PSII activity of mung bean plants.
    • Salience, Myopia, and Complex Dynamic Incentives: Evidence from Medicare Part D

      Dalton, Christina M; Gowrisankaran, Gautam; Town, Robert J; Univ Arizona (OXFORD UNIV PRESS, 2020-03)
      The standard Medicare Part D drug insurance contract is non-linear-with reduced subsidies in a coverage gap-resulting in a dynamic purchase problem. We consider enrolees who arrived near the gap early in the year and show that they should expect to enter the gap with high probability, implying that, under a benchmark model with neoclassical preferences, the gap should impact them very little. We find that these enrolees have flat spending in a period before the doughnut hole and a large spending drop in the gap, providing evidence against the benchmark model. We structurally estimate behavioural dynamic drug purchase models and find that a price salience model where enrolees do not incorporate future prices into their decision-making at all fits the data best. For a nationally representative sample, full price salience would decrease enrolee spending by 31%. Entirely eliminating the gap would increase insurer spending 27%, compared to 7% for generic-drug-only gap coverage.
    • Salinomycin and its derivatives as potent RET transcriptional inhibitors for the treatment of medullary thyroid carcinoma

      Alqahtani, Tariq; Kumarasamy, Vishnu Muthuraj; Huczyński, Adam; Sun, Daekyu; Univ Arizona, Coll Pharm, Dept Pharmacol & Toxicol; Univ Arizona, BIO5 Inst; Univ Arizona, Arizona Canc Ctr (SPANDIDOS PUBL LTD, 2019-11-20)
      Rearranged during transfection kinase (RET) is a validated molecular target in medullary thyroid cancer (MTC), as activating mutations in RET are often associated with the development of MTC. The present study reports the first preclinical characterization of salinomycin and selected analogs as potent RET transcriptional inhibitors. Reverse transcription‑PCR and immunoblotting revealed that salinomycin profoundly decreased RET expression in the TT human MTC cell line by inhibiting RET transcription. Moreover, salinomycin resulted in remarkable anti‑proliferative activity against MTC that is driven by RET (gain of function mutation) by selectively inhibiting the intracellular PI3K/Akt/mTOR signaling pathway. Also, flow cytometry and fluorescence‑activated cell sorting showed that salinomycin induces G1 phase arrest and apoptosis by reducing the expression of retinoblastoma protein, E2F1, cyclin D and CDK4. The structure‑activity relationship of salinomycin was investigated in this study. Some of the salinomycin derivatives showed the ability to reduce RET expression where others fail to alter RET expression. These results suggest that the RET‑suppressing effect of salinomycin may be largely attributed to disruption of the Wnt pathway, presumably through interference with the ternary LRP6‑Frizzled‑Wnt complex. Furthermore, these findings support the further preclinical evaluation of salinomycin and its analogs as a promising new class of therapeutic agents for the improved treatment of MTC.
    • Salutary effects of moderate but not high intensity aerobic exercise training on the frequency of peripheral T-cells associated with immunosenescence in older women at high risk of breast cancer: a randomized controlled trial

      Niemiro, G.M.; Coletta, A.M.; Agha, N.H.; Mylabathula, P.L.; Baker, F.L.; Brewster, A.M.; Bevers, T.B.; Fuentes-Mattei, E.; Basen-Engquist, K.; Katsanis, E.; et al. (BioMed Central Ltd, 2022)
      Background: Immunosenescence is described as age-associated changes within the immune system that are responsible for decreased immunity and increased cancer risk. Physically active individuals have fewer ‘senescent’ and more naïve T-cells compared to their sedentary counterparts, but it is not known if exercise training can rejuvenate ‘older looking’ T-cell profiles. We determined the effects of 12-weeks supervised exercise training on the frequency of T-cell subtypes in peripheral blood and their relationships with circulating levels of the muscle-derived cytokines (i.e. ‘myokines’) IL-6, IL-7, IL-15 and osteonectin in older women at high risk of breast cancer. The intervention involved 3 sessions/week of either high intensity interval exercise (HIIT) or moderate intensity continuous exercise (MICT) and were compared to an untrained control (UC) group. Results: HIIT decreased total granulocytes, CD4+ T-cells, CD4+ naïve T-cells, CD4+ recent thymic emigrants (RTE) and the CD4:CD8 ratio after training, whereas MICT increased total lymphocytes and CD8 effector memory (EM) T-cells. The change in total T-cells, CD4+ naïve T-cells, CD4+ central memory (CM) T-cells and CD4+ RTE was elevated after MICT compared to HIIT. Changes in V ̇ O 2 max after training, regardless of exercise prescription, was inversely related to the change in highly differentiated CD8+ EMRA T-cells and positively related to changes in β2-adrenergic receptor (β2-AR) expression on CM CD4+ and CM CD8+ T-cells. Plasma myokine levels did not change significantly among the groups after training, but individual changes in IL-7 were positively related to changes in the number of β2-AR expressing CD4 naïve T cells in both exercise groups but not controls. Further, CD4 T-cells and CD4 naive T-cells were negatively related to changes in IL-6 and osteonectin after HIIT but not MICT, whereas CD8 EMRA T-cells were inversely related to changes in IL-15 after MICT but not HIIT. Conclusions: Aerobic exercise training alters the frequency of peripheral T-cells associated with immunosenescence in middle aged/older women at high risk of breast cancer, with HIIT (pro-senescent) and MICT (anti-senescent) evoking divergent effects. Identifying the underlying mechanisms and establishing whether exercise-induced changes in peripheral T-cell numbers can alter the risk of developing breast cancer warrants investigation. © 2022, The Author(s).
    • Salvage Hyperthermic Gemcitabine and Docetaxel Combination Chemotherapy After BCG Failure in Non-Muscle Invasive Bladder Cancer Patients

      Rao, Mounica Y.; Kang, Paul; Tarajkowski, Jamaka C.; Mobley, Debra L.; Lamm, Donald L.; Univ Arizona, Coll Med (IOS PRESS, 2020-03-28)
      BACKGROUND: Bacillus Calmette-Guerin (BCG) is the recommended therapy for high and intermediate risk non-muscle invasive bladder cancer (NMIBC), but treatment failure is common. While a radical cystectomy is recommended after BCG failure, some patients desire bladder preservation and others are poor surgical candidates. Salvage chemotherapy treatments may be offered to this subgroup of patients. OBJECTIVE: To assess if combination, hyperthermic Gemcitabine and Docetaxel chemotherapy (GEM/DOCE) is a safe and effective salvage option for treating NMIBC. METHODS: Sixty patients who received our GEM/DOCE protocol between 2007-2017 were identified (51 BCG failures, 9 BCG naive). This study measured overall treatment success, defined as no recurrence, progression, cystectomy, nor death due to bladder cancer. Kaplan-Meier curves were used to ascertain probability of treatment success. The log-rank test was used to identify factors associated with treatment success. RESULTS: Sixty patients received treatment with a median follow-up of 14.9 months. All patients completed the induction course with no significant adverse effects. Overall treatment success was 83% at first surveillance, 69% at 1 year, and 55% at 2 years in the entire cohort, and 90% at first surveillance, 74% at 1 year, and 56% at 2 years in the BCG-failure patients. All-cause and bladder-cancer-specific survival were both 97.9% at 1 year, 85.9% and 94.6% respectively at 2 years. Three patients underwent cystectomy at a median of 10.2 months, two of these were secondary to recurrences. Three patients had progression of their disease. CONCLUSIONS: Hyperthermic GEM/DOCE seems to be a well-tolerated salvage regimen that demonstrates a reasonable efficacy and warrants further investigation.
    • Salvage Stereotactic Body Radiation Therapy for Locally Recurrent Previously Irradiated Head and Neck Squamous Cell Carcinoma: An Analysis from the RSSearch® Registry

      Ansinelli, Hayden; Singh, Raj; Sharma, Dana L; Jenkins, Jan; Davis, Joanne; Vargo, John A; Sharma, Sanjeev; Univ Arizona, Dept Radiat Oncol, Coll Med (CUREUS INC, 2018-08-31)
      Objectives To report on overall survival (OS), local control (LC), dose-outcome relationships, and related toxicities following stereotactic body radiation therapy (SBRT) for locally recurrent, previously irradiated squamous cell carcinoma of the head and neck (rSCCHN). Methods We queried the prospectively-maintained RSSearch (R) Registry for patients with rSCCHN treated with five-fraction SBRT from January 2008 to November 2016. Patients with non-squamous cell histology, missing registry data regarding prior irradiation, those treated with less than five fractions of SBRT, and those treated with SBRT in primary or boost settings were excluded. LC and OS were estimated using the Kaplan-Meier method with comparisons between groups completed using log-rank t-tests and multivariable Cox regression. Logistic regression analyses were used to examine factors predictive of toxicity. Results Forty-five rSCCHN patients treated with SBRT delivered in five fractions at 12 radiotherapy centers were identified. Prescription doses >= 40 Gy were associated with higher one-year rates of OS, LC, and a higher likelihood of experiencing toxicities. Acute and late toxicity rates were low (22.2% and 15.6%, respectively) and were all Grade 1-2 with only one late Grade 3 esophagitis. Conclusion Salvage SBRT for rSCCHN resulted in outcomes comparable to prior single-institutional reports in a multi-institutional cohort across clinical settings with low toxicity, thus supporting more widespread adoption of SBRT with recommended doses >= 40 Gy.
    • Salvia palmetorum (Lamiaceae), a new species from Sonora, Mexico

      González-Gallegos, Jesús Guadalupe; Carnahan, Susan D.; Univ Arizona, Herbarium (INST BIOLOGIA, UNIV NACIONAL AUTONOMA MEXICO, 2019-10-03)
      A new Salvia L. species was discovered in the vicinity of San Carlos Bay (Guaymas county) in Sonora, Mexico. The discovery was carried out during botanical explorations for the floristic inventory of the area. The new taxon is described and illustrated. This is an interesting addition to the Flora of Sonora because it constitutes the first Salvia endemic to this state. The species is characterized by an unusual combination of characters within Salvia subg. Calosphace (Benth.) Epling: densely white-tomentulose stems, presence of bracteoles, flowers arranged in thyrses, trimucronate upper calyx lip and ornate connective with an antrorse obtuse tooth. The taxon is also atypical among Mexican Salvia subg. Calosphace in growing in palm groves below 100 m elevation.
    • Sample identifiers and metadata to support data management and reuse in multidisciplinary ecosystem sciences

      Damerow, J.E.; Varadharajan, C.; Boye, K.; Brodie, E.L.; Burrus, M.; Chadwick, K.D.; Crystal-Ornelas, R.; Elbashandy, H.; Eloy Alves, R.J.; Ely, K.S.; et al. (Ubiquity Press, 2021)
      Physical samples are foundational entities for research across biological, Earth, and environmental sciences. Data generated from sample-based analyses are not only the basis of individual studies, but can also be integrated with other data to answer new and broader-scale questions. Ecosystem studies increasingly rely on multidisciplinary team-science to study climate and environmental changes. While there are widely adopted conventions within certain domains to describe sample data, these have gaps when applied in a multidisciplinary context. In this study, we reviewed existing practices for identifying, characterizing, and linking related environmental samples. We then tested practicalities of assigning persistent identifiers to samples, with standardized metadata, in a pilot field test involving eight United States Department of Energy projects. Participants collected a variety of sample types, with analyses conducted across multiple facilities. We address terminology gaps for multidisciplinary research and make recommendations for assigning identifiers and metadata that supports sample tracking, integration, and reuse. Our goal is to provide a practical approach to sample management, geared towards ecosystem scientists who contribute and reuse sample data. (IGSN); physical samples; soil; water; plant; leaf; microbial communities; related identifiers; persistent identifiers. © 2021 The Author(s).
    • Sample preparation method for metal(loid) contaminant quantitation in rodent hair collected in Yuma County, Arizona

      Credo, Jonathan; Chandos, Amy; Checinski, Camilla; von Hippel, Frank A.; Ingram, Jani C.; College of Medicine, University of Arizona; Community, Environment and Policy, Mel and Enid Zuckerman College of Public Health, University of Arizona (Springer Science and Business Media LLC, 2021-07-27)
      Yuma County, Arizona, is a large agricultural hub of the USA located in the southwestern corner of Arizona on the USA-Mexico border. Year-round use of agrichemicals at a massive scale along with the influx of aquatic contaminants in the Colorado River led to significant levels of environmental pollution and hence exposure risks for people and wildlife. Although hair is a recognized biomarker for metal exposure, there is no universal hair preparation protocol. This study evaluated two digestion methods for metal quantitation using inductively coupled plasma-mass spectrometry (ICP-MS) and three methods for mercury quantitation using cold vapor-atomic absorption spectroscopy (CV-AAS), both employing certified reference materials. The “overnight” and “heating” digestion methods were suitable for ICP-MS, while only the heating method was suitable for CV-AAS. These validated methods will be useful for a variety of human and wildlife assessments of toxic metal(loid) exposure.
    • Sampling density and date along with species selection influence spatial representation of tree-ring reconstructions

      Maxwell, Justin T.; Harley, Grant L.; Matheus, Trevis J.; Strange, Brandon M.; Van Aken, Kayla; Au, Tsun Fung; Bregy, Joshua C.; Univ Arizona, Sch Nat Resources & Environm (COPERNICUS GESELLSCHAFT MBH, 2020-10-19)
      Our understanding of the natural variability of hydroclimate before the instrumental period (ca. 1900 CE in the United States) is largely dependent on tree-ring-based reconstructions. Large-scale soil moisture reconstructions from a network of tree-ring chronologies have greatly improved our understanding of the spatial and temporal variability in hydroclimate conditions, particularly extremes of both drought and pluvial (wet) events. However, certain regions within these large-scale network reconstructions in the US are modeled by few tree-ring chronologies. Further, many of the chronologies currently publicly available on the International Tree-Ring Data Bank (ITRDB) were collected in the 1980s and 1990s, and thus our understanding of the sensitivity of radial growth to soil moisture in the US is based on a period that experienced multiple extremely severe droughts and neglects the impacts of recent, rapid global change. In this study, we expanded the tree-ring network of the Ohio River valley in the US, a region with sparse coverage. We used a total of 72 chronologies across 15 species to examine how increasing the density of the tree-ring network influences the representation of reconstructing the Palmer Meteorological Drought Index (PMDI). Further, we tested how the sampling date and therefore the calibration period influenced the reconstruction models by creating reconstructions that ended in the year 1980 and compared them to reconstructions ending in 2010 from the same chronologies. We found that in- creasing the density of the tree-ring network resulted in reconstructed values that better matched the spatial variability of instrumentally recorded droughts and, to a lesser extent, pluvials. By extending the calibration period to 2010 compared to 1980, the sensitivity of tree rings to PMDI decreased in the southern portion of our region where severe drought conditions have been absent over recent decades. We emphasize the need of building a high-density tree-ring network to better represent the spatial variability of past droughts and pluvials. Further, chronologies on the ITRDB need updating regularly to better understand how the sensitivity of tree rings to climate may vary through time.