Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomics
Affiliation
Arizona Genomics Institute, Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USADepartment of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
Benaroya Research Institute at Virginia Mason, 1201 Ninth Avenue, Seattle, WA 98101, USA
Department of Biology and Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA
Department of Biology and Center for Developmental Biology, University of Washington, Seattle, WA 98195, USA
Department of Genetics, Biochemistry and Life Science Studies, Clemson University, Clemson, SC 29634, USA
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA
Issue Date
2005Metadata
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BioMed CentralCitation
BMC Plant Biology 2005, 5:10 doi:10.1186/1471-2229-5-10Journal
BMC Plant BiologyRights
© 2005 Wang et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)Collection Information
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.Abstract
BACKGROUND:The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants.RESULTS:Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clonesthe average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3% of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes.CONCLUSION:The S. moellenforffii BAC library, the first to be constructed from a lycophyte, will be useful to the scientific community as a resource for comparative plant genomics and evolution.