Show simple item record

Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomics

dc.contributor.authorWang, Wenming
dc.contributor.authorTanurdzic, Milos
dc.contributor.authorLuo, Meizhong
dc.contributor.authorSisneros, Nicholas
dc.contributor.authorKim, Hye Ran
dc.contributor.authorWeng, Jing-Ke
dc.contributor.authorKudrna, Dave
dc.contributor.authorMueller, Christopher
dc.contributor.authorArumuganathan, K.
dc.contributor.authorCarlson, John
dc.contributor.authorChapple, Clint
dc.contributor.authorde Pamphilis, Claude
dc.contributor.authorMandoli, Dina
dc.contributor.authorTomkins, Jeff
dc.contributor.authorWing, Rod
dc.contributor.authorBanks, Jo Ann
dc.date.accessioned2016-05-20T08:58:03Z
dc.date.available2016-05-20T08:58:03Z
dc.date.issued2005en
dc.identifier.citationBMC Plant Biology 2005, 5:10 doi:10.1186/1471-2229-5-10en
dc.identifier.doi10.1186/1471-2229-5-10en
dc.identifier.urihttp://hdl.handle.net/10150/610078
dc.description.abstractBACKGROUND:The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants.RESULTS:Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clones
dc.description.abstractthe average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3% of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes.CONCLUSION:The S. moellenforffii BAC library, the first to be constructed from a lycophyte, will be useful to the scientific community as a resource for comparative plant genomics and evolution.
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2229/5/10en
dc.rights© 2005 Wang et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleConstruction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomicsen
dc.typeArticleen
dc.identifier.eissn1471-2229en
dc.contributor.departmentArizona Genomics Institute, Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USAen
dc.contributor.departmentDepartment of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USAen
dc.contributor.departmentDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USAen
dc.contributor.departmentBenaroya Research Institute at Virginia Mason, 1201 Ninth Avenue, Seattle, WA 98101, USAen
dc.contributor.departmentDepartment of Biology and Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USAen
dc.contributor.departmentDepartment of Biology and Center for Developmental Biology, University of Washington, Seattle, WA 98195, USAen
dc.contributor.departmentDepartment of Genetics, Biochemistry and Life Science Studies, Clemson University, Clemson, SC 29634, USAen
dc.contributor.departmentCold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USAen
dc.contributor.departmentCenter for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USAen
dc.identifier.journalBMC Plant Biologyen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
refterms.dateFOA2018-09-11T10:45:21Z
html.description.abstractBACKGROUND:The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants.RESULTS:Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clones
html.description.abstractthe average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3% of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes.CONCLUSION:The S. moellenforffii BAC library, the first to be constructed from a lycophyte, will be useful to the scientific community as a resource for comparative plant genomics and evolution.


Files in this item

Thumbnail
Name:
1471-2229-5-10.pdf
Size:
1.907Mb
Format:
PDF
Download

This item appears in the following Collection(s)

Show simple item record