Spatial distribution of transcript changes in the maize primary root elongation zone at low water potential
Author
Spollen, WilliamTao, Wenjing
Valliyodan, Babu
Chen, Kegui
Hejlek, Lindsey
Kim, Jong-Joo
LeNoble, Mary
Zhu, Jinming
Bohnert, Hans
Henderson, David
Schachtman, Daniel
Davis, Georgia
Springer, Gordon
Sharp, Robert
Nguyen, Henry
Affiliation
Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USADepartment of Animal Science, University of Arizona, Tucson, Arizona 85721, USA
Department of Computer Science, University of Missouri, Columbia, MO 65211, USA
Department of Plant Biology and Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
W. M. Keck Center for Comparative and Functional Genomics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
Donald Danforth Plant Science Center, St. Louis, Missouri 63132, USA
School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 712749 South Korea
Research Support Computing, University of Missouri, Columbia, MO 65211, USA
Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547, USA
School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 712749 South Korea
Insightful Corporation, Seattle, WA 98109, USA
Issue Date
2008
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BioMed CentralCitation
BMC Plant Biology 2008, 8:32 doi:10.1186/1471-2229-8-32Journal
BMC Plant BiologyRights
© 2008 Spollen et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0).Collection Information
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.Abstract
BACKGROUND:Previous work showed that the maize primary root adapts to low Psiw (-1.6 MPa) by maintaining longitudinal expansion in the apical 3 mm (region 1), whereas in the adjacent 4 mm (region 2) longitudinal expansion reaches a maximum in well-watered roots but is progressively inhibited at low Psiw. To identify mechanisms that determine these responses to low Psiw, transcript expression was profiled in these regions of water-stressed and well-watered roots. In addition, comparison between region 2 of water-stressed roots and the zone of growth deceleration in well-watered roots (region 3) distinguished stress-responsive genes in region 2 from those involved in cell maturation.RESULTS:Responses of gene expression to water stress in regions 1 and 2 were largely distinct. The largest functional categories of differentially expressed transcripts were reactive oxygen species and carbon metabolism in region 1, and membrane transport in region 2. Transcripts controlling sucrose hydrolysis distinguished well-watered and water-stressed states (invertase vs. sucrose synthase), and changes in expression of transcripts for starch synthesis indicated further alteration in carbon metabolism under water deficit. A role for inositols in the stress response was suggested, as was control of proline metabolism. Increased expression of transcripts for wall-loosening proteins in region 1, and for elements of ABA and ethylene signaling were also indicated in the response to water deficit.CONCLUSION:The analysis indicates that fundamentally different signaling and metabolic response mechanisms are involved in the response to water stress in different regions of the maize primary root elongation zone.EISSN
1471-2229Version
Final published versionAdditional Links
http://www.biomedcentral.com/1471-2229/8/32ae974a485f413a2113503eed53cd6c53
10.1186/1471-2229-8-32
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Except where otherwise noted, this item's license is described as © 2008 Spollen et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0).