Show simple item record

dc.contributor.authorSpollen, William
dc.contributor.authorTao, Wenjing
dc.contributor.authorValliyodan, Babu
dc.contributor.authorChen, Kegui
dc.contributor.authorHejlek, Lindsey
dc.contributor.authorKim, Jong-Joo
dc.contributor.authorLeNoble, Mary
dc.contributor.authorZhu, Jinming
dc.contributor.authorBohnert, Hans
dc.contributor.authorHenderson, David
dc.contributor.authorSchachtman, Daniel
dc.contributor.authorDavis, Georgia
dc.contributor.authorSpringer, Gordon
dc.contributor.authorSharp, Robert
dc.contributor.authorNguyen, Henry
dc.date.accessioned2016-05-20T08:58:07Z
dc.date.available2016-05-20T08:58:07Z
dc.date.issued2008en
dc.identifier.citationBMC Plant Biology 2008, 8:32 doi:10.1186/1471-2229-8-32en
dc.identifier.doi10.1186/1471-2229-8-32en
dc.identifier.urihttp://hdl.handle.net/10150/610081
dc.description.abstractBACKGROUND:Previous work showed that the maize primary root adapts to low Psiw (-1.6 MPa) by maintaining longitudinal expansion in the apical 3 mm (region 1), whereas in the adjacent 4 mm (region 2) longitudinal expansion reaches a maximum in well-watered roots but is progressively inhibited at low Psiw. To identify mechanisms that determine these responses to low Psiw, transcript expression was profiled in these regions of water-stressed and well-watered roots. In addition, comparison between region 2 of water-stressed roots and the zone of growth deceleration in well-watered roots (region 3) distinguished stress-responsive genes in region 2 from those involved in cell maturation.RESULTS:Responses of gene expression to water stress in regions 1 and 2 were largely distinct. The largest functional categories of differentially expressed transcripts were reactive oxygen species and carbon metabolism in region 1, and membrane transport in region 2. Transcripts controlling sucrose hydrolysis distinguished well-watered and water-stressed states (invertase vs. sucrose synthase), and changes in expression of transcripts for starch synthesis indicated further alteration in carbon metabolism under water deficit. A role for inositols in the stress response was suggested, as was control of proline metabolism. Increased expression of transcripts for wall-loosening proteins in region 1, and for elements of ABA and ethylene signaling were also indicated in the response to water deficit.CONCLUSION:The analysis indicates that fundamentally different signaling and metabolic response mechanisms are involved in the response to water stress in different regions of the maize primary root elongation zone.
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2229/8/32en
dc.rights© 2008 Spollen et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0).en
dc.rights.urihttps://creativecommons.org/licenses/by/2.0/
dc.titleSpatial distribution of transcript changes in the maize primary root elongation zone at low water potentialen
dc.typeArticleen
dc.identifier.eissn1471-2229en
dc.contributor.departmentDivision of Plant Sciences, University of Missouri, Columbia, MO 65211, USAen
dc.contributor.departmentDepartment of Animal Science, University of Arizona, Tucson, Arizona 85721, USAen
dc.contributor.departmentDepartment of Computer Science, University of Missouri, Columbia, MO 65211, USAen
dc.contributor.departmentDepartment of Plant Biology and Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USAen
dc.contributor.departmentW. M. Keck Center for Comparative and Functional Genomics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USAen
dc.contributor.departmentDonald Danforth Plant Science Center, St. Louis, Missouri 63132, USAen
dc.contributor.departmentSchool of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 712749 South Koreaen
dc.contributor.departmentResearch Support Computing, University of Missouri, Columbia, MO 65211, USAen
dc.contributor.departmentBio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547, USAen
dc.contributor.departmentSchool of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 712749 South Koreaen
dc.contributor.departmentInsightful Corporation, Seattle, WA 98109, USAen
dc.identifier.journalBMC Plant Biologyen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
refterms.dateFOA2018-06-23T23:28:51Z
html.description.abstractBACKGROUND:Previous work showed that the maize primary root adapts to low Psiw (-1.6 MPa) by maintaining longitudinal expansion in the apical 3 mm (region 1), whereas in the adjacent 4 mm (region 2) longitudinal expansion reaches a maximum in well-watered roots but is progressively inhibited at low Psiw. To identify mechanisms that determine these responses to low Psiw, transcript expression was profiled in these regions of water-stressed and well-watered roots. In addition, comparison between region 2 of water-stressed roots and the zone of growth deceleration in well-watered roots (region 3) distinguished stress-responsive genes in region 2 from those involved in cell maturation.RESULTS:Responses of gene expression to water stress in regions 1 and 2 were largely distinct. The largest functional categories of differentially expressed transcripts were reactive oxygen species and carbon metabolism in region 1, and membrane transport in region 2. Transcripts controlling sucrose hydrolysis distinguished well-watered and water-stressed states (invertase vs. sucrose synthase), and changes in expression of transcripts for starch synthesis indicated further alteration in carbon metabolism under water deficit. A role for inositols in the stress response was suggested, as was control of proline metabolism. Increased expression of transcripts for wall-loosening proteins in region 1, and for elements of ABA and ethylene signaling were also indicated in the response to water deficit.CONCLUSION:The analysis indicates that fundamentally different signaling and metabolic response mechanisms are involved in the response to water stress in different regions of the maize primary root elongation zone.


Files in this item

Thumbnail
Name:
1471-2229-8-32.pdf
Size:
732.9Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record

© 2008 Spollen et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0).
Except where otherwise noted, this item's license is described as © 2008 Spollen et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0).