AffiliationDepartment of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721, USA
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88011, USA
Laboratoire de Génetique Moléculaire des Plantes, UMR CNRS 5575, Université Joseph Fourier, 38041 Grenoble, France
Department of Plant Biology, University of Illinois, Urbana, IL 61801, USA
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CitationGenome Biology 2001, 3(1):research0001.1–0001.17 http://genomebiology.com/2001/3/1/research/0001
Rights© 2001 Quigley et al., licensee BioMed Central Ltd.
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AbstractBACKGROUND:In the post-genomic era newly sequenced genomes can be used to deduce organismal functions from our knowledge of other systems. Here we apply this approach to analyzing the aquaporin gene family in Arabidopsis thaliana. The aquaporins are intrinsic membrane proteins that have been characterized as facilitators of water flux. Originally termed major intrinsic proteins (MIPs), they are now also known as water channels, glycerol facilitators and aqua-glyceroporins, yet recent data suggest that they facilitate the movement of other low-molecular-weight metabolites as well.RESULTS:The Arabidopsis genome contains 38 sequences with homology to aquaporin in four subfamilies, termed PIP, TIP, NIP and SIP. We have analyzed aquaporin family structure and expression using the A. thaliana genome sequence, and introduce a new NMR approach for the purpose of analyzing water movement in plant roots in vivo.CONCLUSIONS:Our preliminary data indicate a strongly transcellular component for the flux of water in roots.
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