PublisherThe University of Arizona.
AbstractThere are many known proteins that are necessary for endocytosis at the plasma membrane. However, little is known about the proteins that are involved in lysosome formation. The Fares lab is interested in identifying novel proteins involved in lysosome formation. A forward genetic screen identified cup-12 with a phenotype that indicated a lysosome defect. Using complementation tests and sequencing, I identified cup-12 as clh-6. clh-6 is one of six ClC-type voltage-gated chloride channels in C. elegans. CLH-6 is most closely related to mammalian ClC6 and ClC7. Chloride channels regulate the excitability of cells, volume control, and organelle acidification. Mutations in chloride channels can lead to diseases such as Dent’s disease and Cystic Fibrosis. Our data suggest that loss of some ClCs causes lysosome dysfunction that could contribute to disease symptoms.