CELLULOSE DEGRADATION IN LUTEIBACTER: SUSPECTED GENETIC MECHANISMS AND BIOCHEMICAL APPLICATIONS
PublisherThe University of Arizona.
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AbstractThe role of Luteibacter as a cellulose degradation microorganism is examined in this thesis. An overview of some of the key functions of cellulose in the biosphere is given as well as a review of cellulolytic strategies and mechanisms employed in the microscopic world. A brief characterization of the genus Luteibacter is also provided. Experiments were aimed at evaluating loss of function cellulase mutants and abnormal phenotypic mutants in order to better characterize potential genetic pathways used by Luteibacter in cellulose degradation. While there is still much work to be done evaluating this system, initial results indicate that the protein GfcC as well as a phosphokinase and a type I secretion system likely play important roles in Luteibacter’s ability to secrete cellulase. Future plasmid sequencing data will likely shed more light on the specific gene regions associated with cellulase production and secretion in Luteibacter. Further understanding of the mechanisms and genetics behind cellulose degradation in Luteibacter will provide key insights into the process of microbial cellulose utilization as a whole, a process which has implications in a wide variety of innovative biological and industrial fields.
Degree ProgramHonors College
Molecular and Cellular Biology