DNA methylation changes facilitated evolution of genes derived from Mutator-like transposable elements
Wing, Rod A.
AffiliationUniv Arizona, Arizona Genom Inst, Inst BIO5
Univ Arizona, Sch Plant Sci
MetadataShow full item record
PublisherBIOMED CENTRAL LTD
CitationDNA methylation changes facilitated evolution of genes derived from Mutator-like transposable elements 2016, 17 (1) Genome Biology
Rights© 2016 Wang et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
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AbstractBackground: Mutator-like transposable elements, a class of DNA transposons, exist pervasively in both prokaryotic and eukaryotic genomes, with more than 10,000 copies identified in the rice genome. These elements can capture ectopic genomic sequences that lead to the formation of new gene structures. Here, based on whole-genome comparative analyses, we comprehensively investigated processes and mechanisms of the evolution of putative genes derived from Mutator-like transposable elements in ten Oryza species and the outgroup Leersia perieri, bridging similar to 20 million years of evolutionary history. Results: Our analysis identified thousands of putative genes in each of the Oryza species, a large proportion of which have evidence of expression and contain chimeric structures. Consistent with previous reports, we observe that the putative Mutator-like transposable element-derived genes are generally GC-rich and mainly derive from GC-rich parental sequences. Furthermore, we determine that Mutator-like transposable elements capture parental sequences preferentially from genomic regions with low methylation levels and high recombination rates. We explicitly show that methylation levels in the internal and terminated inverted repeat regions of these elements, which might be directed by the 24-nucleotide small RNA-mediated pathway, are different and change dynamically over evolutionary time. Lastly, we demonstrate that putative genes derived from Mutator-like transposable elements tend to be expressed in mature pollen, which have undergone de-methylation programming, thereby providing a permissive expression environment for newly formed/transposable element-derived genes. Conclusions: Our results suggest that DNA methylation may be a primary mechanism to facilitate the origination, survival, and regulation of genes derived from Mutator-like transposable elements, thus contributing to the evolution of gene innovation and novelty in plant genomes.
VersionFinal published version
SponsorsThe project was funded by a start-up fund from Wayne State University to CF; RAW was supported by the National Science Foundation Plant Genome Program ( grant number 1026200), the Bud Antle Endowed Chair of Excellence in Agriculture, and the AXA Chair for Evolutionary Genomic and Genome Biology.