Arabidopsis CALCINEURIN B-LIKE10 Functions Independently of the SOS Pathway during Reproductive Development in Saline Conditions.

Monihan, Shea M; Magness, Courtney A; Yadegari, Ramin; Smith, Steven E; Schumaker, Karen S

dc.contributor.author	Monihan, Shea M
dc.contributor.author	Magness, Courtney A
dc.contributor.author	Yadegari, Ramin
dc.contributor.author	Smith, Steven E
dc.contributor.author	Schumaker, Karen S
dc.date.accessioned	2016-07-08T01:06:44Z
dc.date.available	2016-07-08T01:06:44Z
dc.date.issued	2016-05
dc.identifier.citation	Arabidopsis CALCINEURIN B-LIKE10 Functions Independently of the SOS Pathway during Reproductive Development in Saline Conditions. 2016, 171 (1):369-79 Plant Physiol.	en
dc.identifier.issn	1532-2548
dc.identifier.pmid	26979332
dc.identifier.doi	10.1104/pp.16.00334
dc.identifier.uri	http://hdl.handle.net/10150/615779
dc.description.abstract	The accumulation of sodium in soil (saline conditions) negatively affects plant growth and development. The Salt Overly Sensitive (SOS) pathway in Arabidopsis (Arabidopsis thaliana) functions to remove sodium from the cytosol during vegetative development preventing its accumulation to toxic levels. In this pathway, the SOS3 and CALCINEURIN B-LIKE10 (CBL10) calcium sensors interact with the SOS2 protein kinase to activate sodium/proton exchange at the plasma membrane (SOS1) or vacuolar membrane. To determine if the same pathway functions during reproductive development in response to salt, fertility was analyzed in wild type and the SOS pathway mutants grown in saline conditions. In response to salt, CBL10 functions early in reproductive development before fertilization, while SOS1 functions mostly after fertilization when seed development begins. Neither SOS2 nor SOS3 function in reproductive development in response to salt. Loss of CBL10 function resulted in reduced anther dehiscence, shortened stamen filaments, and aborted pollen development. In addition, cbl10 mutant pistils could not sustain the growth of wild-type pollen tubes. These results suggest that CBL10 is critical for reproductive development in the presence of salt and that it functions in different pathways during vegetative and reproductive development.
dc.language.iso	en	en
dc.publisher	AMER SOC PLANT BIOLOGISTS	en
dc.rights	Copyright © 2016 American Society of Plant Biologists. All rights reserved.	en
dc.rights.uri	http://rightsstatements.org/vocab/InC/1.0/
dc.title	Arabidopsis CALCINEURIN B-LIKE10 Functions Independently of the SOS Pathway during Reproductive Development in Saline Conditions.	en
dc.type	Article	en
dc.contributor.department	Univ Arizona, Sch Plant Sci	en
dc.contributor.department	Univ Arizona, Sch Nat Resources & Environm	en
dc.identifier.journal	Plant physiology	en
dc.description.note	Preview published March 15, 2016. 12 month embargo.	en
dc.description.collectioninformation	This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.	en
dc.eprint.version	Final accepted manuscript	en
refterms.dateFOA	2017-03-15T00:00:00Z
html.description.abstract	The accumulation of sodium in soil (saline conditions) negatively affects plant growth and development. The Salt Overly Sensitive (SOS) pathway in Arabidopsis (Arabidopsis thaliana) functions to remove sodium from the cytosol during vegetative development preventing its accumulation to toxic levels. In this pathway, the SOS3 and CALCINEURIN B-LIKE10 (CBL10) calcium sensors interact with the SOS2 protein kinase to activate sodium/proton exchange at the plasma membrane (SOS1) or vacuolar membrane. To determine if the same pathway functions during reproductive development in response to salt, fertility was analyzed in wild type and the SOS pathway mutants grown in saline conditions. In response to salt, CBL10 functions early in reproductive development before fertilization, while SOS1 functions mostly after fertilization when seed development begins. Neither SOS2 nor SOS3 function in reproductive development in response to salt. Loss of CBL10 function resulted in reduced anther dehiscence, shortened stamen filaments, and aborted pollen development. In addition, cbl10 mutant pistils could not sustain the growth of wild-type pollen tubes. These results suggest that CBL10 is critical for reproductive development in the presence of salt and that it functions in different pathways during vegetative and reproductive development.