• Login
    View Item 
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Master's Theses
    • View Item
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Master's Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA Catalogs

    Statistics

    Display statistics

    Investigation of the Feasibility of an Optical Imaging System for the Application of In Vivo Flow Cytometry

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    azu_etd_14994_sip1_m.pdf
    Size:
    2.398Mb
    Format:
    PDF
    Download
    Author
    Martin, Phillip A.
    Issue Date
    2016
    Keywords
    Detection
    Flow
    Fluorescence
    OTL38
    Signal
    Optical Sciences
    Cytometry
    Advisor
    Gmitro, Arthur
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    This thesis investigates the feasibility of employing an optical imaging system for the application of in vivo flow cytometry for detecting rare circulating tumor cells (CTCs) in vasculature. This investigation presented used three optical imaging configurations: a Nikon Eclipse E600 fluorescence microscope with a PIXIS 2048B CCD camera; a Nikon Eclipse E600 fluorescence microscope with a ThorLabs DCC 3240N CMOS camera; and a custom built confocal microendoscope with a ThorLabs DCC 3240N CMOS camera. These systems were employed to gain insight as to what signal to noise ratios and sensitivities are required to sufficiently detect fluorescently labeled cancer cells. This work presents general concepts of fluorescence and confocal microscopy, the experimental setups employed, and experimental measurements and results obtained. The experimental measurements involved the following: the simulation of flow cytometry by imaging green fluorescent microspheres, with a fluorescence excitation range of 505-515 nm and a diameter of 15µm, in a square crit tube moving on a translational stage, and imaging a selection of cells that included MCF10A breast cells (non-cancerous), OVCAR3 ovarian cancer cells, and patient derived xenogram (PDX) breast cancer cells, which express folate-receptor proteins on their surface. We fluorescently labeled these cells with the introduction of a new folate-receptor targeted fluorescent contrast agent OTL38, made by On Target Laboratories. The results established that we were able to image and detect fluorescence microspheres with a minimum signal to noise ratio (SNR) of 2.3 using the ThorLabs DCC 3240N camera on the Nikon Fluorescence microscope. We were able to image and detect the cells used on all three system configurations. Analyzing the different cell uptake efficacies of the contrast agent OTL38, we established that the SNR levels were variable when imaging PDX breast cancer cells. We propose future work to investigate possible effects on the variability of SNR results, as well as, and future steps in designing a real-time optical fluorescence imaging system for in vivo flow cytometry.
    Type
    text
    Electronic Thesis
    Degree Name
    M.S.
    Degree Level
    masters
    Degree Program
    Graduate College
    Optical Sciences
    Degree Grantor
    University of Arizona
    Collections
    Master's Theses

    entitlement

     
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.