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dc.contributor.authorLi, Xianchun*
dc.contributor.authorWang, Ling*
dc.contributor.authorWan, Peng*
dc.contributor.authorCong, Shengbo*
dc.contributor.authorWang, Jintao*
dc.contributor.authorHuang, Minsong*
dc.contributor.authorTabashnik, Bruce E.*
dc.contributor.authorWu, Kongming*
dc.date.accessioned2016-11-10T04:06:52Z
dc.date.available2016-11-10T04:06:52Z
dc.date.issued2016-06
dc.identifier.citationWang, Ling, Peng Wan, Shengbo Cong, Jintao Wang, Minsong Huang, Bruce E. Tabashnik, Xianchun Li, and Kongming Wu. "Adult Exposure to Bt Toxin Cry1Ac Reduces Life Span and Reproduction of Resistant and Susceptible Pink Bollworm (Lepidoptera: Gelechiidae)." Journal of economic entomology 109, no. 3 (2016): 1357-1363.en
dc.identifier.issn0022-0493
dc.identifier.doi10.1093/jee/tow039
dc.identifier.urihttp://hdl.handle.net/10150/621322
dc.description.abstractInsecticidal proteins from Bacillus thuringiensis (Bt) are used widely in sprays and transgenic plants to control insect pests. Although much research has elucidated the effects of Bt toxins on larvae, relatively little is known about their effects on adults. Here, we evaluated the effects of exposing adults to Bt toxin Cry1Ac on the life span and reproduction of two strains of pink bollworm (Pectinophora gossypiella (Saunders)). In larval diet bioassays, the concentration of Cry1Ac killing 50% of larvae (LC50) was 640 times higher for the laboratory-selected resistant strain (AZP-R) than the susceptible strain (APHIS-S). In experiments with adults, the highest concentrations of Cry1Ac tested (160 and 640 mu g Cry1Ac per ml of 5% honey water) reduced life span for both strains. Treatments with 10, 40, and 160 mg Cry1Ac per ml reduced the duration of the oviposition period as well as the number of eggs laid by both strains, but did not affect the percentage of pairs producing eggs, the duration of the preoviposition period, or the percentage of eggs hatching for either strain. Adult life span did not differ between strains at low to moderate concentrations of Cry1Ac, but it was significantly greater for the resistant strain than the susceptible strain at the two highest concentrations of Cry1Ac tested. The reduced susceptibility to high concentrations of Cry1Ac in adults of the AZP-R strain relative to the APHIS-S strain provides the first evidence of expression of resistance to a Bt toxin in adult Lepidoptera.
dc.description.sponsorshipNational Natural Science Foundation of China [30625028, 31321004]en
dc.language.isoenen
dc.publisherOXFORD UNIV PRESS INCen
dc.relation.urlhttp://jee.oxfordjournals.org/content/early/2016/04/30/jee.tow039en
dc.rights© The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved.en
dc.subjectPectinophora gossypiellaen
dc.subjectBacillus thuringiensisen
dc.subjectadult life spanen
dc.subjectreproductionen
dc.titleAdult Exposure to Bt Toxin Cry1Ac Reduces Life Span and Reproduction of Resistant and Susceptible Pink Bollworm (Lepidoptera: Gelechiidae)en
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Dept Entomolen
dc.identifier.journalJOURNAL OF ECONOMIC ENTOMOLOGYen
dc.description.noteFirst published online: 1 May 2016. 12 month embargo.en
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal accepted manuscripten
refterms.dateFOA2017-05-01T00:00:00Z
html.description.abstractInsecticidal proteins from Bacillus thuringiensis (Bt) are used widely in sprays and transgenic plants to control insect pests. Although much research has elucidated the effects of Bt toxins on larvae, relatively little is known about their effects on adults. Here, we evaluated the effects of exposing adults to Bt toxin Cry1Ac on the life span and reproduction of two strains of pink bollworm (Pectinophora gossypiella (Saunders)). In larval diet bioassays, the concentration of Cry1Ac killing 50% of larvae (LC50) was 640 times higher for the laboratory-selected resistant strain (AZP-R) than the susceptible strain (APHIS-S). In experiments with adults, the highest concentrations of Cry1Ac tested (160 and 640 mu g Cry1Ac per ml of 5% honey water) reduced life span for both strains. Treatments with 10, 40, and 160 mg Cry1Ac per ml reduced the duration of the oviposition period as well as the number of eggs laid by both strains, but did not affect the percentage of pairs producing eggs, the duration of the preoviposition period, or the percentage of eggs hatching for either strain. Adult life span did not differ between strains at low to moderate concentrations of Cry1Ac, but it was significantly greater for the resistant strain than the susceptible strain at the two highest concentrations of Cry1Ac tested. The reduced susceptibility to high concentrations of Cry1Ac in adults of the AZP-R strain relative to the APHIS-S strain provides the first evidence of expression of resistance to a Bt toxin in adult Lepidoptera.


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