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dc.contributor.authorMcNamara, James W.
dc.contributor.authorLi, Amy
dc.contributor.authorSmith, Nicola J.
dc.contributor.authorLal, Sean
dc.contributor.authorGraham, Robert M.
dc.contributor.authorKooiker, Kristina Bezold
dc.contributor.authorvan Dijk, Sabine J.
dc.contributor.authorRemedios, Cristobal G. dos
dc.contributor.authorHarris, Samantha P.
dc.contributor.authorCooke, Roger
dc.date.accessioned2016-11-10T04:23:24Z
dc.date.available2016-11-10T04:23:24Z
dc.date.issued2016-05
dc.identifier.citationAblation of cardiac myosin binding protein-C disrupts the super-relaxed state of myosin in murine cardiomyocytes 2016, 94:65 Journal of Molecular and Cellular Cardiologyen
dc.identifier.issn00222828
dc.identifier.pmid27021517
dc.identifier.doi10.1016/j.yjmcc.2016.03.009
dc.identifier.urihttp://hdl.handle.net/10150/621325
dc.description.abstractCardiac myosin binding protein-C (cMyBP-C) is a structural and regulatory component of cardiac thick filaments. It is observed in electron micrographs as seven to nine transverse stripes in the central portion of each half of the A band. Its C-terminus binds tightly to the myosin rod and contributes to thick filament structure, while the N-terminus can bind both myosin S2 and actin, influencing their structure and function. Mutations in the MYBPC3 gene (encoding cMyBP-C) are commonly associated with hypertrophic cardiomyopathy (HCM). In cardiac cells there exists a population of myosin heads in the super-relaxed (SRX) state, which are bound to the thick filament core with a highly inhibited ATPase activity. This report examines the role cMyBP-C plays in regulating the population of the SRX state of cardiac myosin by using an assay that measures single ATP turnover of myosin. We report a significant decrease in the proportion of myosin heads in the SRX state in homozygous cMyBP-C knockout mice, however heterozygous cMyBP-C knockout mice do not significantly differ from the wild type. A smaller, non-significant decrease is observed when thoracic aortic constriction is used to induce cardiac hypertrophy in mutation negative mice. These results support the proposal that cMyBP-C stabilises the thick filament and that the loss of cMyBP-C results in an untethering of myosin heads. This results in an increased myosin ATP turnover, further consolidating the relationship between thick filament structure and the myosin ATPase. Crown Copyright (C) 2016 Published by Elsevier Ltd. All rights reserved.
dc.description.sponsorshipNG Macintosh Memorial Fund; National Heart Foundation [GNT1093852, PB 12S 6939]; NIH [R01 HL080367]; American Heart Association [13POST14780089]en
dc.language.isoenen
dc.publisherELSEVIER SCI LTDen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0022282816300566en
dc.rightsCrown Copyright © 2016 Published by Elsevier Ltd. All rights reserved.en
dc.subjectCardiac SRXen
dc.subjectHypertrophic cardiomyopathyen
dc.subjectMyosin binding protein-C (MyBP-C)en
dc.subjectMyosin II ATPaseen
dc.subjectThick filament structureen
dc.subjectCardiac energeticsen
dc.titleAblation of cardiac myosin binding protein-C disrupts the super-relaxed state of myosin in murine cardiomyocytesen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Dept Cellular & Mol Meden
dc.identifier.journalJournal of Molecular and Cellular Cardiologyen
dc.description.noteAvailable online 26 March 2016. 12 month embargo.en
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal accepted manuscripten
refterms.dateFOA2017-03-26T00:00:00Z
html.description.abstractCardiac myosin binding protein-C (cMyBP-C) is a structural and regulatory component of cardiac thick filaments. It is observed in electron micrographs as seven to nine transverse stripes in the central portion of each half of the A band. Its C-terminus binds tightly to the myosin rod and contributes to thick filament structure, while the N-terminus can bind both myosin S2 and actin, influencing their structure and function. Mutations in the MYBPC3 gene (encoding cMyBP-C) are commonly associated with hypertrophic cardiomyopathy (HCM). In cardiac cells there exists a population of myosin heads in the super-relaxed (SRX) state, which are bound to the thick filament core with a highly inhibited ATPase activity. This report examines the role cMyBP-C plays in regulating the population of the SRX state of cardiac myosin by using an assay that measures single ATP turnover of myosin. We report a significant decrease in the proportion of myosin heads in the SRX state in homozygous cMyBP-C knockout mice, however heterozygous cMyBP-C knockout mice do not significantly differ from the wild type. A smaller, non-significant decrease is observed when thoracic aortic constriction is used to induce cardiac hypertrophy in mutation negative mice. These results support the proposal that cMyBP-C stabilises the thick filament and that the loss of cMyBP-C results in an untethering of myosin heads. This results in an increased myosin ATP turnover, further consolidating the relationship between thick filament structure and the myosin ATPase. Crown Copyright (C) 2016 Published by Elsevier Ltd. All rights reserved.


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