Independent Co-Option of a Tailed Bacteriophage into a Killing Complex in Pseudomonas
AffiliationSchool of Plant Sciences, University of Arizona
Department of Entomology and Center for Insect Science, University of Arizona
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PublisherAmerican Society for Microbiology
CitationIndependent Co-Option of a Tailed Bacteriophage into a Killing Complex in Pseudomonas 2015, 6 (4):e00452-15 mBio
RightsCopyright © 2015 Hockett et al. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
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AbstractCompetition between microbes is widespread in nature, especially among those that are closely related. To combat competitors, bacteria have evolved numerous protein-based systems (bacteriocins) that kill strains closely related to the producer. In characterizing the bacteriocin complement and killing spectra for the model strain Pseudomonas syringae B728a, we discovered that its activity was not linked to any predicted bacteriocin but is derived from a prophage. Instead of encoding an active prophage, this region encodes a bacteriophage-derived bacteriocin, termed an R-type syringacin. This R-type syringacin is striking in its convergence with the well-studied R-type pyocin of P. aeruginosa in both genomic location and molecular function. Genomic alignment, amino acid percent sequence identity, and phylogenetic inference all support a scenario where the R-type syringacin has been co-opted independently of the R-type pyocin. Moreover, the presence of this region is conserved among several other Pseudomonas species and thus is likely important for intermicrobial interactions throughout this important genus.
DescriptionUA Open Access Publishing Fund
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VersionFinal published version