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dc.contributor.authorLi, Guangbin
dc.contributor.authorSierra-Alvarez, Reyes
dc.contributor.authorVilcherrez, David
dc.contributor.authorWeiss, Stefan
dc.contributor.authorGill, Callie
dc.contributor.authorKrzmarzick, Mark J
dc.contributor.authorAbrell, Leif
dc.contributor.authorField, Jim A.
dc.date.accessioned2016-12-01T23:54:40Z
dc.date.available2016-12-01T23:54:40Z
dc.date.issued2016-10-04
dc.identifier.citationNitrate Reverses Severe Nitrite Inhibition of Anaerobic Ammonium Oxidation (Anammox) Activity in Continuously-Fed Bioreactors 2016, 50 (19):10518 Environmental Science & Technologyen
dc.identifier.issn0013-936X
dc.identifier.issn1520-5851
dc.identifier.doi10.1021/acs.est.6b01560
dc.identifier.urihttp://hdl.handle.net/10150/621475
dc.description.abstractNitrite (NO2-) substrate under certain conditions can cause failure of N-removal processes relying on anaerobic ammonium oxidizing (anammox) bacteria. Detoxification of NO2- can potentially be achieved by using exogenous nitrate (NO3-). In this work, continuous experiments in bioreactors with anammox bacteria closely related to “Candidatus Brocadia caroliniensis” were conducted to evaluate the effectiveness of short NO3- additions to reverse NO2- toxicity. The results show that a timely NO3- addition immediately after a NO2- stress event completely reversed the NO2- inhibition. This reversal occurs without NO3- being metabolized as evidence by lack of any 30N2 formation from 15N-NO3-. The maximum recovery rate was observed with 5 mM NO3- added for 3 days; however, slower but significant recovery was also observed with 5 mM NO3- for 1 day or 2 mM NO3- for 3 days. Without NO3- addition, long-term NO2- inhibition of anammox biomass resulted in irreversible damage of the cells. These results suggest that a short duration dose of NO3- to an anammox bioreactor can rapidly restore the activity of NO2--stressed anammox cells. On the basis of the results, a hypothesis about the detoxification mechanism related to narK genes in anammox bacteria is proposed and discussed.
dc.description.sponsorshipThis work was supported by the University of Arizona Water Sustainability Program, and the National Science Foundation (Contract CBET-1234211)en
dc.language.isoenen
dc.publisherAMER CHEMICAL SOCen
dc.relation.urlhttp://pubs.acs.org/doi/abs/10.1021/acs.est.6b01560en
dc.rights© 2016 American Chemical Societyen
dc.subjectNitrogen removalen
dc.subjectNitrite inhibitionen
dc.subjectNitrateen
dc.subjectContinuous bioreactorsen
dc.subjectDetoxificationen
dc.titleNitrate Reverses Severe Nitrite Inhibition of Anaerobic Ammonium Oxidation (Anammox) Activity in Continuously-Fed Bioreactorsen
dc.typeArticleen
dc.contributor.departmentUniversity of Arizonaen
dc.identifier.journalEnvironmental Science & Technologyen
dc.description.notePublication Date (Web): September 6, 2016. 12 month embargo.en
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal accepted manuscripten
refterms.dateFOA2017-09-06T00:00:00Z
html.description.abstractNitrite (NO2-) substrate under certain conditions can cause failure of N-removal processes relying on anaerobic ammonium oxidizing (anammox) bacteria. Detoxification of NO2- can potentially be achieved by using exogenous nitrate (NO3-). In this work, continuous experiments in bioreactors with anammox bacteria closely related to “Candidatus Brocadia caroliniensis” were conducted to evaluate the effectiveness of short NO3- additions to reverse NO2- toxicity. The results show that a timely NO3- addition immediately after a NO2- stress event completely reversed the NO2- inhibition. This reversal occurs without NO3- being metabolized as evidence by lack of any 30N2 formation from 15N-NO3-. The maximum recovery rate was observed with 5 mM NO3- added for 3 days; however, slower but significant recovery was also observed with 5 mM NO3- for 1 day or 2 mM NO3- for 3 days. Without NO3- addition, long-term NO2- inhibition of anammox biomass resulted in irreversible damage of the cells. These results suggest that a short duration dose of NO3- to an anammox bioreactor can rapidly restore the activity of NO2--stressed anammox cells. On the basis of the results, a hypothesis about the detoxification mechanism related to narK genes in anammox bacteria is proposed and discussed.


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