Use of Bacillus spp. to enhance phosphorus availability and serve as a plant growth promoter in aquaponics systems

Abstract

Plant growth promoters (PGP) are microorganisms essential for sustainable food production systems by improving the productivity of crops and mitigating environmental impacts. Microorganisms enhance the P availability to plants by mineralizing organic P and solubilizing precipitated phosphates. This work is focused on the effect of inoculation of a commercial product containing a mixture of Bacillus spp. on hydroponically grown lettuce (Lactuca sativa) integrated with tilapia (Orechromis niloticus) aquaculture in a closed-loop system, in comparison with an untreated control. We determined plant growth and crop quality parameters to assess the efficacy of the beneficial microorganisms. A nutrient dynamics analysis was conducted to evaluate the effect of Bacillus inoculation on the changes of nutrient concentration in aquaponics solutions, as well as the phosphorus accumulation in several components (fish, plants, water and solids). We performed a plate-count assay to quantify the number microorganisms present in systems inoculated or not with the commercial Bacillus mixture. In general, nutrient dynamics was affected by the inclusion of the Bacillus mixture in the water. Systems that received the product showed faster decreases in ammonia concentration and faster increase in nitrite and nitrate concentrations than the control. The untreated aquaponics systems showed lower accumulation of phosphorus in the water than systems receiving the Bacillus mixture, which resulted in poor plant growth, low phosphorus accumulation in the leaves and low chlorophyll content. However, the mass balance analysis showed that an external source of phosphorus possibly contributed to the overall P budget in systems receiving the Bacillus mixture. The microbial plate count assay demonstrated an active microbiota in aquaponics systems receiving the treatment while untreated systems showed zero microbial counts. The Bacillus mixture used in the present study appears to have PGP properties and to affect P dynamics in aquaponics systems. However, since the product contained traces of phosphorus in its composition, further analysis will be necessary to distinguish whether the advantageous effects promoted by the Bacillus occurred as a result of a beneficial microbial activity or a fertilizing effect. (C) 2016 Elsevier B.V. All rights reserved.