Carbon monoxide attenuates the effects of snake venoms containing metalloproteinases with fibrinogenase or thrombin-like activity on plasmatic coagulation
AffiliationUniv Arizona, Coll Med, Dept Anesthesiol
MetadataShow full item record
PublisherROYAL SOC CHEMISTRY
CitationCarbon monoxide attenuates the effects of snake venoms containing metalloproteinases with fibrinogenase or thrombin-like activity on plasmatic coagulation 2016, 7 (10):1973 Med. Chem. Commun.
JournalMed. Chem. Commun.
RightsThis article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractExposure of plasma to iron and carbon monoxide (CO) renders fibrinogen resistant to fibrinogenolytic or thrombin-like activity contained in pit viper venom. However, the direct effects of iron/CO on venom activity are unknown. Thus, we assessed if four different, metalloproteinase containing snake venoms exposed to iron/CO or CO alone could attenuate their fibrinogenolytic or thrombin-like activity. Venom (0-500 mu g ml(-1)) was exposed to 0-10 mu M FeCl3 and/or 0-100 mu M carbon monoxide releasing molecule-2 (CORM-2), or inactivated CORM-2 (iCORM-2) for 3 min at room temperature. Venom solution (0-8 mu g ml(-1) final concentration) was then placed in citrated human plasma containing tissue factor, followed by CaCl2 addition for commencement of coagulation. Data were determined with thrombelastography for 10-15 min at 37 degrees C. Iron had no effect on the first venom tested, so only CO was investigated subsequently. Exposure of venom to CO attenuated fibrinogenolytic or thrombin-like activity, and iCORM-2 did not affect the venom activities. Further investigation of the effect of CO exposure on similar venoms is justified.
NoteOpen access article-RSC Gold.
VersionFinal published version
SponsorsDepartment of Anesthesiology