A centrosome interactome provides insight into organelle assembly and reveals a non-duplication role for Plk4
AuthorGalletta, Brian J.
Fagerstrom, Carey J.
Schoborg, Todd A.
McLamarrah, Tiffany A.
Ryniawec, John M.
Buster, Daniel W.
Slep, Kevin C.
Rogers, Gregory C.
Rusan, Nasser M.
AffiliationUniv Arizona, Ctr Canc, Dept Cellular & Mol Med
MetadataShow full item record
PublisherNATURE PUBLISHING GROUP
CitationA centrosome interactome provides insight into organelle assembly and reveals a non-duplication role for Plk4 2016, 7:12476 Nature Communications
RightsThis work is licensed under a Creative Commons Attribution 4.0 International License.
Collection Information© The Author(s) 2016. This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractThe centrosome is the major microtubule-organizing centre of many cells, best known for its role in mitotic spindle organization. How the proteins of the centrosome are accurately assembled to carry out its many functions remains poorly understood. The non-membranebound nature of the centrosome dictates that protein-protein interactions drive its assembly and functions. To investigate this massive macromolecular organelle, we generated a `domain-level' centrosome interactome using direct protein-protein interaction data from a focused yeast two-hybrid screen. We then used biochemistry, cell biology and the model organism Drosophila to provide insight into the protein organization and kinase regulatory machinery required for centrosome assembly. Finally, we identified a novel role for Plk4, the master regulator of centriole duplication. We show that Plk4 phosphorylates Cep135 to properly position the essential centriole component Asterless. This interaction landscape affords a critical framework for research of normal and aberrant centrosomes.
VersionFinal published version
SponsorsDivision of Intramural Research at the NIH/NHLBI [1ZIAHL006104]; NIH/NIGMS [R01GM110166, R01GM094415]; NSF [MCB1158151]