Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability
AuthorWeegman, Bradley P.
Kumar Sajja, Venkata Sunil
Suszynski, Thomas M.
Rizzari, Michael D.
Scott III, William E.
Kitzmann, Jennifer P.
Mueller, Kate R.
Hanley, Thomas R.
Kennedy, David J.
Todd, Paul W.
Balamurugan, Appakalai N.
Hering, Bernhard J.
Papas, Klearchos K.
AffiliationUniv Arizona, Dept Surg
MetadataShow full item record
PublisherHINDAWI PUBLISHING CORP
CitationContinuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability 2016, 2016:1 Journal of Diabetes Research
JournalJournal of Diabetes Research
Rights© 2016 Bradley P. Weegman et al. This is an open access article distributed under the Creative Commons Attribution License.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractIslet transplantation (ITx) is an emerging and promising therapy for patients with uncontrolled type 1 diabetes. The islet isolation and purification processes require exposure to extended cold ischemia, warm-enzymatic digestion, mechanical agitation, and use of damaging chemicals for density gradient separation (DG), all of which reduce viable islet yield. In this paper, we describe initial proof-of-concept studies exploring quadrupole magnetic separation (QMS) of islets as an alternative to DG to reduce exposure to these harsh conditions. Three porcine pancreata were split into two parts, the splenic lobe (SPL) and the combined connecting/duodenal lobes (CDL), for paired digestions and purifications. Islets in the SPL were preferentially labeled using magnetic microparticles (MMPs) that lodge within the islet microvasculature when infused into the pancreas and were continuously separated from the exocrine tissue by QMS during the collection phase of the digestion process. Unlabeled islets from the CDL were purified by conventional DG. Islets purified by QMS exhibited significantly improved viability (measured by oxygen consumption rate per DNA, p < 0.03 ) and better morphology relative to control islets. Islet purification by QMS can reduce the detrimental effects of prolonged exposure to toxic enzymes and density gradient solutions and substantially improve islet viability after isolation.
VersionFinal published version
SponsorsSchulze Foundation; Schulze Diabetes Institute, NIH [R44DK072647-03, R01DK068717]; Carol Olson Memorial Diabetes Research Fund; Iacocca Family Foundation; Schott Family Foundation; Kettering Family Foundation