Early and Persistent Dendritic Hypertrophy in the Basolateral Amygdala following Experimental Diffuse Traumatic Brain Injury
AuthorHoffman, Ann N.
Paode, Pooja R.
May, Hazel G.
Ortiz, J. Bryce
Conrad, Cheryl D.
Currier Thomas, Theresa
AffiliationDepartment of Child Health, University of Arizona College of Medicine-Phoenix
MetadataShow full item record
PublisherMARY ANN LIEBERT, INC
CitationEarly and Persistent Dendritic Hypertrophy in the Basolateral Amygdala following Experimental Diffuse Traumatic Brain Injury 2017, 34 (1):213 Journal of Neurotrauma
JournalJournal of Neurotrauma
Rights© Mary Ann Liebert, Inc.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractIn the pathophysiology of traumatic brain injury (TBI), the amygdala remains understudied, despite involvement in processing emotional and stressful stimuli associated with anxiety disorders, such as post-traumatic stress disorder (PTSD). Because the basolateral amygdala (BLA) integrates inputs from sensory and other limbic structures coordinating emotional learning and memory, injury-induced changes in circuitry may contribute to psychiatric sequelae of TBI. This study quantified temporal changes in dendritic complexity of BLA neurons after experimental diffuse TBI, modeled by midline fluid percussion injury. At post-injury days (PIDs) 1, 7, and 28, brain tissue from sham and brain-injured adult, male rats was processed for Golgi, glial fibrillary acidic protein (GFAP), or silver stain and analyzed to quantify BLA dendritic branch intersections, activated astrocytes, and regional neuropathology, respectively. Compared to sham, brain-injured rats at all PIDs showed enhanced dendritic branch intersections in both pyramidal and stellate BLA neuronal types, as evidenced by Sholl analysis. GFAP staining in the BLA was significantly increased at PID1 and 7 in comparison to sham. However, the BLA was relatively spared from neuropathology, demonstrated by an absence of argyrophilic accumulation over time, in contrast to other brain regions. These data suggest an early and persistent enhancement of dendritic complexity within the BLA after a single diffuse TBI. Increased dendritic complexity would alter information processing into and through the amygdala, contributing to emotional symptoms post-TBI, including PTSD.
NoteOnline Ahead of Print: July 27, 2016; 12 month embargo.
VersionFinal accepted manuscript
SponsorsArizona Biomedical Research Commission through the Arizona Department of Health Services [ADHS14-00003606]; National Institute of Neurological Disorders and Stroke of the National Institutes of Health [R01 NS-065052]; Phoenix Children's Hospital