An unrecognized function for COPII components in recruiting the viral replication protein BMV 1a to the perinuclear ER
AffiliationUniv Arizona, Dept Pharmacol & Toxicol
Viral replication protein targeting
Protein perinuclear ER localization
Positive-strand RNA viruses
Viral replication complexes
MetadataShow full item record
PublisherCOMPANY OF BIOLOGISTS LTD
CitationAn unrecognized function for COPII components in recruiting the viral replication protein BMV 1a to the perinuclear ER 2016, 129 (19):3597 Journal of Cell Science
JournalJournal of Cell Science
Rights© 2016. Published by The Company of Biologists Ltd
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractPositive-strand RNAviruses invariably assemble their viral replication complexes (VRCs) by remodeling host intracellular membranes. How viral replication proteins are targeted to specific organelle membranes to initiate VRC assembly remains elusive. Brome mosaic virus (BMV), whose replication can be recapitulated in Saccharomyces cerevisiae, assembles its VRCs by invaginating the outer perinuclear endoplasmic reticulum (ER) membrane. Remarkably, BMV replication protein 1a (BMV 1a) is the only viral protein required for such membrane remodeling. We show that ER-vesicle protein of 14 kD (Erv14), a cargo receptor of coat protein complex II (COPII), interacts with BMV 1a. Moreover, the perinuclear ER localization of BMV 1a is disrupted in cells lacking ERV14 or expressing dysfunctional COPII coat components (Sec13, Sec24 or Sec31). The requirement of Erv14 for the localization of BMV 1a is bypassed by addition of a Sec24-recognizable sorting signal to BMV 1a or by overexpressing Sec24, suggesting a coordinated effort by both Erv14 and Sec24 for the proper localization of BMV 1a. The COPII pathway is well known for being involved in protein secretion; our data suggest that a subset of COPII coat proteins have an unrecognized role in targeting proteins to the perinuclear ER membrane.
NotePublished online October 1, 2016; 12 month embargo
VersionFinal published version
SponsorsUS-Israel Binational Science Foundation (BSF) ; Virginia Tech Startup; National Science Foundation (NSF) 
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