Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum
Publisher
NATURE PUBLISHING GROUPCitation
Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum 2016, 6:31693 Scientific ReportsJournal
Scientific ReportsRights
© The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies.ISSN
2045-2322PubMed ID
27528413Version
Final published versionSponsors
Stanton Foundation; Molecular Life Science initiative of the University of Oslo (MLSuio); Norwegian Research CouncilAdditional Links
http://www.nature.com/articles/srep31693Scopus Count
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Except where otherwise noted, this item's license is described as © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License.