Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum
| dc.contributor.author | Brandtzaeg, Ole Kristian | |
| dc.contributor.author | Johnsen, Elin | |
| dc.contributor.author | Roberg-Larsen, Hanne | |
| dc.contributor.author | Seip, Knut Fredrik | |
| dc.contributor.author | MacLean, Evan L. | |
| dc.contributor.author | Gesquiere, Laurence R. | |
| dc.contributor.author | Leknes, Siri | |
| dc.contributor.author | Lundanes, Elsa | |
| dc.contributor.author | Wilson, Steven Ray | |
| dc.date.accessioned | 2017-03-02T19:25:52Z | |
| dc.date.available | 2017-03-02T19:25:52Z | |
| dc.date.issued | 2016-08-16 | |
| dc.identifier.citation | Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum 2016, 6:31693 Scientific Reports | en |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.pmid | 27528413 | |
| dc.identifier.doi | 10.1038/srep31693 | |
| dc.identifier.uri | http://hdl.handle.net/10150/622705 | |
| dc.description.abstract | The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies. | |
| dc.description.sponsorship | Stanton Foundation; Molecular Life Science initiative of the University of Oslo (MLSuio); Norwegian Research Council | en |
| dc.language.iso | en | en |
| dc.publisher | NATURE PUBLISHING GROUP | en |
| dc.relation.url | http://www.nature.com/articles/srep31693 | en |
| dc.rights | © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. | en |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.title | Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum | en |
| dc.type | Article | en |
| dc.contributor.department | Univ Arizona, Sch Anthropol | en |
| dc.identifier.journal | Scientific Reports | en |
| dc.description.collectioninformation | This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu. | en |
| dc.eprint.version | Final published version | en |
| refterms.dateFOA | 2018-04-26T01:49:13Z | |
| html.description.abstract | The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies. |
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