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    Zebrafish Video Analysis System for High-Throughput Drug Assay

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    azu_etd_15016_sip1_m.pdf
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    Author
    Todd, Douglas Wallace
    Issue Date
    2016
    Keywords
    zebrafish
    image analysis
    Advisor
    Rodríguez, Jeffrey J.
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Embargo
    Release after 13-Jul-2017
    Abstract
    Zebrafish swimming behavior is used in a new, automated drug assay system as a biomarker to measure drug efficiency to prevent or restore hearing loss. This system records video of zebrafish larvae under infrared lighting using Raspberry Pi cameras and measures fish swimming behavior. This automated system significantly reduces the operator time required to process experiments in parallel. Multiple tanks, each consisting of sixteen experiments are operated in parallel. Once a set of experiments starts, all data transfer and processing operations are automatic. A web interface allows the operator to configure, monitor and control the experiments and review reports. Ethernet connects the various hardware components, allowing loose coupling of the distributed software used to schedule and run the experiments. The operator can configure the data processing to be done on the local computer or offloaded to a high-performance computer cluster to achieve even higher throughput. Computationally efficient image processing algorithms provided automated zebrafish detection and motion analysis. Quantitative assessment of error in the position and orientation of the detected fish uses manual data analysis by human observers as the reference. The system error in orientation and position is comparable to human inter-operator error.
    Type
    text
    Electronic Thesis
    Degree Name
    M.S.
    Degree Level
    masters
    Degree Program
    Graduate College
    Electrical & Computer Engineering
    Degree Grantor
    University of Arizona
    Collections
    Master's Theses

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