An Endohyphal Bacterium (Chitinophaga, Bacteroidetes) Alters Carbon Source Use by Fusarium keratoplasticum (F. solani Species Complex, Nectriaceae)
Affiliation
Univ Arizona, Sch Plant SciUniv Arizona, Dept Agr & Biosyst Engn
Univ Arizona, Sch Nat Resources & Environm
Univ Arizona, Dept Ecol & Evolutionary Biol
Issue Date
2017-03-14
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FRONTIERS MEDIA SACitation
An Endohyphal Bacterium (Chitinophaga, Bacteroidetes) Alters Carbon Source Use by Fusarium keratoplasticum (F. solani Species Complex, Nectriaceae) 2017, 8 Frontiers in MicrobiologyJournal
Frontiers in MicrobiologyRights
© 2017 Shaffer, U'Ren, Gallery, Baltrus and Arnold. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Bacterial endosymbionts occur in diverse fungi, including members of many lineages of Ascomycota that inhabit living plants. These endosymbiotic bacteria (endohyphal bacteria, EHB) often can be removed from living fungi by antibiotic treatment, providing an opportunity to assess their effects on functional traits of their fungal hosts. We examined the effects of an endohyphal bacterium (Chitinophaga sp., Bacteroidetes) on substrate use by its host, a seed-associated strain of the fungus Fusarium keratoplasticum, by comparing growth between naturally infected and cured fungal strains across 95 carbon sources with a Biolog((R)) phenotypic microarray. Across the majority of substrates (62%), the strain harboring the bacterium significantly outperformed the cured strain as measured by respiration and hyphal density. These substrates included many that are important for plant-and seed fungus interactions, such as D-trehalose, myoinositol, and sucrose, highlighting the potential influence of EHB on the breadth and efficiency of substrate use by an important Fusariurn species. Cases in which the cured strain outperformed the strain harboring the bacterium were observed in only 5% of substrates. We propose that additive or synergistic substrate use by the fungus bacterium pair enhances fungal growth in this association. More generally, alteration of the breadth or efficiency of substrate use by dispensable EHB may change fungal niches in short timeframes, potentially shaping fungal ecology and the outcomes of fungal-host interactions.Note
Open Access Journal.ISSN
1664-302XPubMed ID
28382021Version
Final published versionSponsors
National Science Foundation [NSF DEB-1119758, NSF DEB-1120205, NSF 10S1354219]; Smithsonian Tropical Research Institute (STRI); Mycological Society of America (Forest Fungal Ecology Award); Graduate and Professional Student Council (Research Award); School of Plant Sciences at The University of Arizona; School of Plant Sciences and College of Agriculture and Life Sciences at the University of ArizonaAdditional Links
http://journal.frontiersin.org/article/10.3389/fmicb.2017.00350/fullae974a485f413a2113503eed53cd6c53
10.3389/fmicb.2017.00350
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Except where otherwise noted, this item's license is described as © 2017 Shaffer, U'Ren, Gallery, Baltrus and Arnold. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
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