An Endohyphal Bacterium (Chitinophaga, Bacteroidetes) Alters Carbon Source Use by Fusarium keratoplasticum (F. solani Species Complex, Nectriaceae)
AffiliationUniv Arizona, Sch Plant Sci
Univ Arizona, Dept Agr & Biosyst Engn
Univ Arizona, Sch Nat Resources & Environm
Univ Arizona, Dept Ecol & Evolutionary Biol
MetadataShow full item record
PublisherFRONTIERS MEDIA SA
CitationAn Endohyphal Bacterium (Chitinophaga, Bacteroidetes) Alters Carbon Source Use by Fusarium keratoplasticum (F. solani Species Complex, Nectriaceae) 2017, 8 Frontiers in Microbiology
JournalFrontiers in Microbiology
Rights© 2017 Shaffer, U'Ren, Gallery, Baltrus and Arnold. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractBacterial endosymbionts occur in diverse fungi, including members of many lineages of Ascomycota that inhabit living plants. These endosymbiotic bacteria (endohyphal bacteria, EHB) often can be removed from living fungi by antibiotic treatment, providing an opportunity to assess their effects on functional traits of their fungal hosts. We examined the effects of an endohyphal bacterium (Chitinophaga sp., Bacteroidetes) on substrate use by its host, a seed-associated strain of the fungus Fusarium keratoplasticum, by comparing growth between naturally infected and cured fungal strains across 95 carbon sources with a Biolog((R)) phenotypic microarray. Across the majority of substrates (62%), the strain harboring the bacterium significantly outperformed the cured strain as measured by respiration and hyphal density. These substrates included many that are important for plant-and seed fungus interactions, such as D-trehalose, myoinositol, and sucrose, highlighting the potential influence of EHB on the breadth and efficiency of substrate use by an important Fusariurn species. Cases in which the cured strain outperformed the strain harboring the bacterium were observed in only 5% of substrates. We propose that additive or synergistic substrate use by the fungus bacterium pair enhances fungal growth in this association. More generally, alteration of the breadth or efficiency of substrate use by dispensable EHB may change fungal niches in short timeframes, potentially shaping fungal ecology and the outcomes of fungal-host interactions.
NoteOpen Access Journal.
VersionFinal published version
SponsorsNational Science Foundation [NSF DEB-1119758, NSF DEB-1120205, NSF 10S1354219]; Smithsonian Tropical Research Institute (STRI); Mycological Society of America (Forest Fungal Ecology Award); Graduate and Professional Student Council (Research Award); School of Plant Sciences at The University of Arizona; School of Plant Sciences and College of Agriculture and Life Sciences at the University of Arizona
- Absence of genome reduction in diverse, facultative endohyphal bacteria.
- Authors: Baltrus DA, Dougherty K, Arendt KR, Huntemann M, Clum A, Pillay M, Palaniappan K, Varghese N, Mikhailova N, Stamatis D, Reddy TBK, Ngan CY, Daum C, Shapiro N, Markowitz V, Ivanova N, Kyrpides N, Woyke T, Arnold AE
- Issue date: 2017 Feb
- Isolation of Endohyphal Bacteria from Foliar Ascomycota and In Vitro Establishment of Their Symbiotic Associations.
- Authors: Arendt KR, Hockett KL, Araldi-Brondolo SJ, Baltrus DA, Arnold AE
- Issue date: 2016 May 15
- Diverse bacteria inhabit living hyphae of phylogenetically diverse fungal endophytes.
- Authors: Hoffman MT, Arnold AE
- Issue date: 2010 Jun
- Endohyphal bacterium enhances production of indole-3-acetic acid by a foliar fungal endophyte.
- Authors: Hoffman MT, Gunatilaka MK, Wijeratne K, Gunatilaka L, Arnold AE
- Issue date: 2013
- Bacterial Endosymbionts: Master Modulators of Fungal Phenotypes.
- Authors: Araldi-Brondolo SJ, Spraker J, Shaffer JP, Woytenko EH, Baltrus DA, Gallery RE, Arnold AE
- Issue date: 2017 Sep