Computational On-Chip Imaging of Nanoparticles and Biomolecules using Ultraviolet Light
AuthorDaloglu, Mustafa Ugur
AffiliationUniv Arizona, Coll Optic Sci
MetadataShow full item record
PublisherNATURE PUBLISHING GROUP
CitationComputational On-Chip Imaging of Nanoparticles and Biomolecules using Ultraviolet Light 2017, 7:44157 Scientific Reports
RightsThis work is licensed under a Creative Commons Attribution 4.0 International License.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at email@example.com.
AbstractSignificant progress in characterization of nanoparticles and biomolecules was enabled by the development of advanced imaging equipment with extreme spatial-resolution and sensitivity. To perform some of these analyses outside of well-resourced laboratories, it is necessary to create robust and cost-effective alternatives to existing high-end laboratory-bound imaging and sensing equipment. Towards this aim, we have designed a holographic on-chip microscope operating at an ultraviolet illumination wavelength (UV) of 266 nm. The increased forward scattering from nanoscale objects at this short wavelength has enabled us to detect individual sub-30 nm nanoparticles over a large field-of- view of > 16 mm(2) using an on-chip imaging platform, where the sample is placed at <= 0.5 mm away from the active area of an opto-electronic sensor-array, without any lenses in between. The strong absorption of this UV wavelength by biomolecules including nucleic acids and proteins has further enabled high-contrast imaging of nanoscopic aggregates of biomolecules, e.g., of enzyme Cu/Zn-superoxide dismutase, abnormal aggregation of which is linked to amyotrophic lateral sclerosis (ALS)-a fatal neurodegenerative disease. This UV-based wide-field computational imaging platform could be valuable for numerous applications in biomedical sciences and environmental monitoring, including disease diagnostics, viral load measurements as well as air-and water-quality assessment.
VersionFinal published version
SponsorsArmy Research Office (ARO) [W911NF-13-1-0419, W911NF-13-1-0197]; ARO Life Sciences Division; National Science Foundation (NSF) CBET Division Biophotonics Program; NSF Emerging Frontiers in Research and Innovation (EFRI) Award; NSF EAGER Award; NSF INSPIRE Award; NSF Partnerships for Innovation; Building Innovation Capacity (PFI: BIC) Program; Office of Naval Research (ONR); National Institutes of Health (NIH); Howard Hughes Medical Institute (HHMI); Vodafone Americas Foundation; Vodafone Americas Foundation, the Mary Kay Foundation; Steven & Alexandra Cohen Foundation; KAUST; RGK Foundation ; National Science Foundation ; American Recovery and Reinvestment Act of 2009 (ARRA)
CollectionsUA Faculty Publications
- Computational imaging of sperm locomotion.
- Authors: Daloglu MU, Ozcan A
- Issue date: 2017 Aug 1
- Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications.
- Authors: Mudanyali O, Tseng D, Oh C, Isikman SO, Sencan I, Bishara W, Oztoprak C, Seo S, Khademhosseini B, Ozcan A
- Issue date: 2010 Jun 7
- Lensfree holographic imaging for on-chip cytometry and diagnostics.
- Authors: Seo S, Su TW, Tseng DK, Erlinger A, Ozcan A
- Issue date: 2009 Mar 21
- Toward giga-pixel nanoscopy on a chip: a computational wide-field look at the nano-scale without the use of lenses.
- Authors: McLeod E, Luo W, Mudanyali O, Greenbaum A, Ozcan A
- Issue date: 2013 Jun 7
- High-throughput screening of large volumes of whole blood using structured illumination and fluorescent on-chip imaging.
- Authors: Arpali SA, Arpali C, Coskun AF, Chiang HH, Ozcan A
- Issue date: 2012 Dec 7