Aflatoxin-free transgenic maize using host-induced gene silencing
AffiliationUniv Arizona, Sch Plant Sci, BIO5 Inst
Univ Arizona, USDA, ARS
Univ Arizona, Sch Plant Sci
MetadataShow full item record
PublisherAMER ASSOC ADVANCEMENT SCIENCE
CitationAflatoxin-free transgenic maize using host-induced gene silencing 2017, 3 (3):e1602382 Science Advances
RightsCopyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
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AbstractAflatoxins, toxic secondary metabolites produced by some Aspergillus species, are a universal agricultural economic problem and a critical health issue. Despite decades of control efforts, aflatoxin contamination is responsible for a global loss of millions of tons of crops each year. We show that host-induced gene silencing is an effective method for eliminating this toxin in transgenic maize. We transformed maize plants with a kernel-specific RNA interference (RNAi) gene cassette targeting the aflC gene, which encodes an enzyme in the Aspergillus aflatoxin biosynthetic pathway. After pathogen infection, aflatoxin could not be detected in kernels from these RNAi transgenic maize plants, while toxin loads reached thousands of parts per billion in nontransgenic control kernels. A comparison of transcripts in developing aflatoxin-free transgenic kernels with those from nontransgenic kernels showed no significant differences between these two groups. These results demonstrate that small interfering RNA molecules can be used to silence aflatoxin biosynthesis in maize, providing an attractive and precise engineering strategy that could also be extended to other crops to improve food security.
NoteOpen Access Journal.
VersionFinal published version
SponsorsBill and Melinda Gates Foundation
Except where otherwise noted, this item's license is described as Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
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