Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam
Affiliation
Univ Arizona, Coll Opt SciUniv Arizona, Dept Mol & Cell Biol
Issue Date
2017-01-04Metadata
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OPTICAL SOC AMERCitation
Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam 2017, 8 (2):570 Biomedical Optics ExpressJournal
Biomedical Optics ExpressRights
© 2017 Optical Society of America.Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 mu m because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 mu m. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos. (C) 2017 Optical Society of AmericaNote
Open Access Journal.ISSN
2156-70852156-7085