Tissue-resident macrophages can contain replication-competent virus in antiretroviral-naive, SIV-infected Asian macaques
AuthorDiNapoli, Sarah R.
Ortiz, Alexandra M.
Twigg, Homer L.
Hirsch, Vanessa M.
Brenchley, Jason M.
AffiliationUniv Arizona, Dept Med
MetadataShow full item record
PublisherAMER SOC CLINICAL INVESTIGATION INC
CitationTissue-resident macrophages can contain replication-competent virus in antiretroviral-naive, SIV-infected Asian macaques 2017, 2 (4) JCI Insight
RightsCopyright © 2017, American Society for Clinical Investigation
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractSIV DNA can be detected in lymphoid tissue-resident macrophages of chronically SIV-infected Asian macaques. These macrophages also contain evidence of recently phagocytosed SIV-infected CD4(+) T cells. Here, we examine whether these macrophages contain replication-competent virus, whether viral DNA can be detected in tissue-resident macrophages from antiretroviral (ARV) therapy-treated animals and humans, and how the viral sequences amplified from macrophages and contemporaneous CD4(+) T cells compare. In ARV-naive animals, we find that lymphoid tissue-resident macrophages contain replication-competent virus if they also contain viral DNA in ARV-naive Asian macaques. The genetic sequence of the virus within these macrophages is similar to those within CD4(+) T cells from the same anatomic sites. In ARV-treated animals, we find that viral DNA can be amplified from lymphoid tissue-resident macrophages of SIV-infected Asian macaques that were treated with ARVs for at least 5 months, but we could not detect replicationcompetent virus from macrophages of animals treated with ARVs. Finally, we could not detect viral DNA in alveolar macrophages from HIV-infected individuals who received ARVs for 3 years and had undetectable viral loads. These data demonstrate that macrophages can contain replicationcompetent virus, but may not represent a significant reservoir for HIV in vivo.
NoteAuthors can deposit to institutional repositories, and we request that they deposit the final, published JCI Insight version.
VersionFinal published version
SponsorsDivision of Intramural Research/NIAID/NIH
- Brain Macrophages in Simian Immunodeficiency Virus-Infected, Antiretroviral-Suppressed Macaques: a Functional Latent Reservoir.
- Authors: Avalos CR, Abreu CM, Queen SE, Li M, Price S, Shirk EN, Engle EL, Forsyth E, Bullock BT, Mac Gabhann F, Wietgrefe SW, Haase AT, Zink MC, Mankowski JL, Clements JE, Gama L
- Issue date: 2017 Aug 15
- Resting CD4+ T lymphocytes but not thymocytes provide a latent viral reservoir in a simian immunodeficiency virus-Macaca nemestrina model of human immunodeficiency virus type 1-infected patients on highly active antiretroviral therapy.
- Authors: Shen A, Zink MC, Mankowski JL, Chadwick K, Margolick JB, Carruth LM, Li M, Clements JE, Siliciano RF
- Issue date: 2003 Apr
- Myeloid and CD4 T Cells Comprise the Latent Reservoir in Antiretroviral Therapy-Suppressed SIVmac251-Infected Macaques.
- Authors: Abreu CM, Veenhuis RT, Avalos CR, Graham S, Parrilla DR, Ferreira EA, Queen SE, Shirk EN, Bullock BT, Li M, Metcalf Pate KA, Beck SE, Mangus LM, Mankowski JL, Mac Gabhann F, O'Connor SL, Gama L, Clements JE
- Issue date: 2019 Aug 20
- Brain macrophages harbor latent, infectious simian immunodeficiency virus.
- Authors: Abreu C, Shirk EN, Queen SE, Beck SE, Mangus LM, Pate KAM, Mankowski JL, Gama L, Clements JE
- Issue date: 2019 Dec 1
- HIV Persistence in Adipose Tissue Reservoirs.
- Authors: Couturier J, Lewis DE
- Issue date: 2018 Feb