Tissue-resident macrophages can contain replication-competent virus in antiretroviral-naive, SIV-infected Asian macaques
AuthorDiNapoli, Sarah R.
Ortiz, Alexandra M.
Twigg, Homer L.
Hirsch, Vanessa M.
Brenchley, Jason M.
AffiliationUniv Arizona, Dept Med
MetadataShow full item record
PublisherAMER SOC CLINICAL INVESTIGATION INC
CitationTissue-resident macrophages can contain replication-competent virus in antiretroviral-naive, SIV-infected Asian macaques 2017, 2 (4) JCI Insight
RightsCopyright © 2017, American Society for Clinical Investigation
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractSIV DNA can be detected in lymphoid tissue-resident macrophages of chronically SIV-infected Asian macaques. These macrophages also contain evidence of recently phagocytosed SIV-infected CD4(+) T cells. Here, we examine whether these macrophages contain replication-competent virus, whether viral DNA can be detected in tissue-resident macrophages from antiretroviral (ARV) therapy-treated animals and humans, and how the viral sequences amplified from macrophages and contemporaneous CD4(+) T cells compare. In ARV-naive animals, we find that lymphoid tissue-resident macrophages contain replication-competent virus if they also contain viral DNA in ARV-naive Asian macaques. The genetic sequence of the virus within these macrophages is similar to those within CD4(+) T cells from the same anatomic sites. In ARV-treated animals, we find that viral DNA can be amplified from lymphoid tissue-resident macrophages of SIV-infected Asian macaques that were treated with ARVs for at least 5 months, but we could not detect replicationcompetent virus from macrophages of animals treated with ARVs. Finally, we could not detect viral DNA in alveolar macrophages from HIV-infected individuals who received ARVs for 3 years and had undetectable viral loads. These data demonstrate that macrophages can contain replicationcompetent virus, but may not represent a significant reservoir for HIV in vivo.
NoteAuthors can deposit to institutional repositories, and we request that they deposit the final, published JCI Insight version.
VersionFinal published version
SponsorsDivision of Intramural Research/NIAID/NIH
- Bone marrow-derived CD4<sup>+</sup> T cells are depleted in SIV-infected macaques and contribute to the size of the replication competent reservoir on ART.
- Authors: Hoang TN, Harper JL, Pino MC, Wang H, Micci L, King CT, McGary CS, McBrien JB, Cervasi B, Silvestri G, Paiardini M
- Issue date: 2018 Oct 10
- Resting CD4+ T lymphocytes but not thymocytes provide a latent viral reservoir in a simian immunodeficiency virus-Macaca nemestrina model of human immunodeficiency virus type 1-infected patients on highly active antiretroviral therapy.
- Authors: Shen A, Zink MC, Mankowski JL, Chadwick K, Margolick JB, Carruth LM, Li M, Clements JE, Siliciano RF
- Issue date: 2003 Apr
- HIV Persistence in Adipose Tissue Reservoirs.
- Authors: Couturier J, Lewis DE
- Issue date: 2018 Feb
- Tenofovir treatment augments anti-viral immunity against drug-resistant SIV challenge in chronically infected rhesus macaques.
- Authors: Metzner KJ, Binley JM, Gettie A, Marx P, Nixon DF, Connor RI
- Issue date: 2006 Dec 21
- Replication-competent simian immunodeficiency virus (SIV) Gag escape mutations archived in latent reservoirs during antiretroviral treatment of SIV-infected macaques.
- Authors: Queen SE, Mears BM, Kelly KM, Dorsey JL, Liao Z, Dinoso JB, Gama L, Adams RJ, Zink MC, Clements JE, Kent SJ, Mankowski JL
- Issue date: 2011 Sep