Tissue-resident macrophages can contain replication-competent virus in antiretroviral-naive, SIV-infected Asian macaques
AuthorDiNapoli, Sarah R.
Ortiz, Alexandra M.
Twigg, Homer L.
Hirsch, Vanessa M.
Brenchley, Jason M.
AffiliationUniv Arizona, Dept Med
MetadataShow full item record
PublisherAMER SOC CLINICAL INVESTIGATION INC
CitationTissue-resident macrophages can contain replication-competent virus in antiretroviral-naive, SIV-infected Asian macaques 2017, 2 (4) JCI Insight
RightsCopyright © 2017, American Society for Clinical Investigation
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractSIV DNA can be detected in lymphoid tissue-resident macrophages of chronically SIV-infected Asian macaques. These macrophages also contain evidence of recently phagocytosed SIV-infected CD4(+) T cells. Here, we examine whether these macrophages contain replication-competent virus, whether viral DNA can be detected in tissue-resident macrophages from antiretroviral (ARV) therapy-treated animals and humans, and how the viral sequences amplified from macrophages and contemporaneous CD4(+) T cells compare. In ARV-naive animals, we find that lymphoid tissue-resident macrophages contain replication-competent virus if they also contain viral DNA in ARV-naive Asian macaques. The genetic sequence of the virus within these macrophages is similar to those within CD4(+) T cells from the same anatomic sites. In ARV-treated animals, we find that viral DNA can be amplified from lymphoid tissue-resident macrophages of SIV-infected Asian macaques that were treated with ARVs for at least 5 months, but we could not detect replicationcompetent virus from macrophages of animals treated with ARVs. Finally, we could not detect viral DNA in alveolar macrophages from HIV-infected individuals who received ARVs for 3 years and had undetectable viral loads. These data demonstrate that macrophages can contain replicationcompetent virus, but may not represent a significant reservoir for HIV in vivo.
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VersionFinal published version
SponsorsDivision of Intramural Research/NIAID/NIH