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dc.contributor.authorWoo, Ho-Hyung*
dc.contributor.authorLee, Sang C.*
dc.contributor.authorGibson, Steven J.*
dc.contributor.authorChambers, Setsuko K.*
dc.date.accessioned2017-05-17T19:07:36Z
dc.date.available2017-05-17T19:07:36Z
dc.date.issued2017-03
dc.identifier.citationExpression of the cytoplasmic nucleolin for post-transcriptional regulation of macrophage colony-stimulating factor mRNA in ovarian and breast cancer cells 2017, 1860 (3):337 Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanismsen
dc.identifier.issn18749399
dc.identifier.doi10.1016/j.bbagrm.2017.01.006
dc.identifier.urihttp://hdl.handle.net/10150/623521
dc.description.abstractThe formation of the mRNP complex is a critical component of translational regulation and mRNA decay. Both the 5 ' and 3 ' UTRs of CSF-1 mRNA are involved in post-transcriptional regulation. In CSF-1 mRNA, a small hairpin loop structure is predicted to form at the extreme 5 ' end (2-21 nt) of the 5 ' UTR. Nucleolin binds the hairpin loop structure in the 5 ' UTR of CSF-1 mRNA and enhances translation, while removal of this hairpin loop nucleolin binding element dramatically represses translation. Thus in CSF-1 mRNA, the hairpin loop nucleolin binding element is critical for translational regulation. In addition, nucleolin interacts with the 3 ' UTR of CSF-1 mRNA and facilitates the miRISC formation which results in poly (A) tail shortening. The overexpression of nucleolin increases the association of CSF-1 mRNA containing short poly (A)(n), <= 26, with polyribosomes. Nucleolin both forms an mRNP complex with the eIF4G and CSF-1 mRNA, and is co-localized with the eIF4G in the cytoplasm further supporting nucleolin's role in translational regulation. The distinct foci formation of nucleolin in the cytoplasm of ovarian and breast cancer cells implicates the translational promoting role of nucleolin in these cancers.
dc.description.sponsorshipWomen's Cancers of the University of Arizona Cancer Center; University of Arizona Cancer Center [P30 CA023074]; Bobbi Olson Endowment Funden
dc.language.isoenen
dc.publisherELSEVIER SCIENCE BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S1874939916302206en
dc.rights© 2017 Elsevier B.V. All rights reserved.en
dc.subjectCytoplasmic nucleolinen
dc.subjectUntranslated regionsen
dc.subjectHairpin loop structureen
dc.subjectelF4Gen
dc.subjectTranslationen
dc.subjectDeadenylationen
dc.subjectMacrophage colony stimulating factor (CSF-1)en
dc.subjectmRNAen
dc.titleExpression of the cytoplasmic nucleolin for post-transcriptional regulation of macrophage colony-stimulating factor mRNA in ovarian and breast cancer cellsen
dc.typeArticleen
dc.contributor.departmentUniversity of Arizona Cancer Centeren
dc.identifier.journalBiochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanismsen
dc.description.note12 month embargo; Available online 25 January 2017en
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal accepted manuscripten
refterms.dateFOA2018-01-26T00:00:00Z
html.description.abstractThe formation of the mRNP complex is a critical component of translational regulation and mRNA decay. Both the 5 ' and 3 ' UTRs of CSF-1 mRNA are involved in post-transcriptional regulation. In CSF-1 mRNA, a small hairpin loop structure is predicted to form at the extreme 5 ' end (2-21 nt) of the 5 ' UTR. Nucleolin binds the hairpin loop structure in the 5 ' UTR of CSF-1 mRNA and enhances translation, while removal of this hairpin loop nucleolin binding element dramatically represses translation. Thus in CSF-1 mRNA, the hairpin loop nucleolin binding element is critical for translational regulation. In addition, nucleolin interacts with the 3 ' UTR of CSF-1 mRNA and facilitates the miRISC formation which results in poly (A) tail shortening. The overexpression of nucleolin increases the association of CSF-1 mRNA containing short poly (A)(n), <= 26, with polyribosomes. Nucleolin both forms an mRNP complex with the eIF4G and CSF-1 mRNA, and is co-localized with the eIF4G in the cytoplasm further supporting nucleolin's role in translational regulation. The distinct foci formation of nucleolin in the cytoplasm of ovarian and breast cancer cells implicates the translational promoting role of nucleolin in these cancers.


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