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    Development of an enzyme immunoassay for detection of antibodies against Coccidioides in dogs and other mammalian species

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    journal.pone.0175081.pdf
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    Author
    Chow, Nancy A. cc
    Lindsley, Mark D.
    McCotter, Orion Z.
    Kangiser, Dave
    Wohrle, Ron D.
    Clifford, Wayne R.
    Yaglom, Hayley D.
    Adams, Laura E.
    Komatsu, Kenneth
    Durkin, Michelle M.
    Baker, Rocky J.
    Shubitz, Lisa F.
    Derado, Gordana
    Chiller, Tom M.
    Litvintseva, Anastasia P.
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    Affiliation
    Univ Arizona, Valley Fever Ctr Excellence
    Issue Date
    2017-04-05
    
    Metadata
    Show full item record
    Publisher
    PUBLIC LIBRARY SCIENCE
    Citation
    Development of an enzyme immunoassay for detection of antibodies against Coccidioides in dogs and other mammalian species 2017, 12 (4):e0175081 PLOS ONE
    Journal
    PLOS ONE
    Rights
    Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    Coccidioides is a soil-dwelling fungus that causes coccidioidomycosis, a disease also known as Valley fever, which affects humans and a variety of animal species. Recent findings of Coccidioides in new, unexpected areas of the United States have demonstrated the need for a better understanding of its geographic distribution. Large serological studies on animals could provide important information on the geographic distribution of this pathogen. To facilitate such studies, we used protein A/G, a recombinant protein that binds IgG antibodies from a variety of mammalian species, to develop an enzyme immunoassay (EIA) that detects IgG antibodies against Coccidioides in a highly sensitive and high-throughput manner. We showed the potential of this assay to be adapted to multiple animal species by testing a collection of serum and/or plasma samples from dogs, mice, and humans with or without confirmed coccidioidomycosis. We then evaluated the performance of the assay in dogs, using sera from dogs residing in a highly endemic area, and found seropositivity rates significantly higher than those in dogs of non-endemic areas. We further evaluated the specificity of the assay in dogs infected with other fungal pathogens known to cross-react with Coccidioides. Finally, we used the assay to perform a cross-sectional serosurvey investigating dogs from Washington, a state in which infection with Coccidioides has recently been documented. In summary, we have developed a Coccidioides EIA for the detection of antibodies in canines that is more sensitive and has higher throughput than currently available methods, and by testing this assay in mice and humans, we have shown a proof of principle of its adaptability for other animal species.
    Note
    Open Access Journal.
    ISSN
    1932-6203
    PubMed ID
    28380017
    DOI
    10.1371/journal.pone.0175081
    Version
    Final published version
    Additional Links
    http://dx.plos.org/10.1371/journal.pone.0175081
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0175081
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