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dc.contributor.authorAydin, Incien
dc.contributor.authorVillalonga-Planells, Ruthen
dc.contributor.authorGreune, Liloen
dc.contributor.authorBronnimann, Matthew P.en
dc.contributor.authorCalton, Christine M.en
dc.contributor.authorBecker, Miriamen
dc.contributor.authorLai, Kun-Yien
dc.contributor.authorCampos, Samuel K.en
dc.contributor.authorSchmidt, M. Alexanderen
dc.contributor.authorSchelhaas, Marioen
dc.date.accessioned2017-07-06T15:54:56Z
dc.date.available2017-07-06T15:54:56Z
dc.date.issued2017-05-02
dc.identifier.citationA central region in the minor capsid protein of papillomaviruses facilitates viral genome tethering and membrane penetration for mitotic nuclear entry 2017, 13 (5):e1006308 PLOS Pathogensen
dc.identifier.issn1553-7374
dc.identifier.doi10.1371/journal.ppat.1006308
dc.identifier.urihttp://hdl.handle.net/10150/624633
dc.description.abstractIncoming papillomaviruses (PVs) depend on mitotic nuclear envelope breakdown to gain initial access to the nucleus for viral transcription and replication. In our previous work, we hypothesized that the minor capsid protein L2 of PVs tethers the incoming vDNA to mitotic chromosomes to direct them into the nascent nuclei. To re-evaluate how dynamic L2 recruitment to cellular chromosomes occurs specifically during prometaphase, we developed a quantitative, microscopy-based assay for measuring the degree of chromosome recruitment of L2-EGFP. Analyzing various HPV16 L2 truncation-mutants revealed a central chromosome-binding region (CBR) of 147 amino acids that confers binding to mitotic chromosomes. Specific mutations of conserved motifs (IVAL286AAAA, RR302/5AA, and RTR313EEE) within the CBR interfered with chromosomal binding. Moreover, assembly-competent HPV16 containing the chromosome-binding deficient L2(RTR313EEE) or L2 (IVAL286AAAA) were inhibited for infection despite their ability to be transported to intracellular compartments. Since vDNA and L2 were not associated with mitotic chromosomes either, the infectivity was likely impaired by a defect in tethering of the vDNA to mitotic chromosomes. However, L2 mutations that abrogated chromatin association also compromised translocation of L2 across membranes of intracellular organelles. Thus, chromatin recruitment of L2 may in itself be a requirement for successful penetration of the limiting membrane thereby linking both processes mechanistically. Furthermore, we demonstrate that the association of L2 with mitotic chromosomes is conserved among the alpha, beta, gamma, and iota genera of Papillomaviridae. However, different binding patterns point to a certain variance amongst the different genera. Overall, our data suggest a common strategy among various PVs, in which a central region of L2 mediates tethering of vDNA to mitotic chromosomes during cell division thereby coordinating membrane translocation and delivery to daughter nuclei.
dc.description.sponsorshipGerman Research Foundation (DFG) [EXC 1003]; Horizon European Research Council (ERC) [CoG - 682899 - MitoVln]; Federal Ministry for Education and Research (BMBF) [031L0095A]; National Institute of Allergy and Infectious Diseases [1R01AI108751-01]en
dc.language.isoenen
dc.publisherPUBLIC LIBRARY SCIENCEen
dc.relation.urlhttp://dx.plos.org/10.1371/journal.ppat.1006308en
dc.rights© 2017 Aydin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.en
dc.titleA central region in the minor capsid protein of papillomaviruses facilitates viral genome tethering and membrane penetration for mitotic nuclear entryen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Dept Immunobiolen
dc.contributor.departmentUniv Arizona, Inst Bio5en
dc.contributor.departmentUniv Arizona, Dept Mol & Cellular Biolen
dc.contributor.departmentUniv Arizona, Canc Biol Grad Interdisciplinary Programen
dc.identifier.journalPLOS Pathogensen
dc.description.noteOpen access journalen
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
refterms.dateFOA2018-06-25T17:38:29Z
html.description.abstractIncoming papillomaviruses (PVs) depend on mitotic nuclear envelope breakdown to gain initial access to the nucleus for viral transcription and replication. In our previous work, we hypothesized that the minor capsid protein L2 of PVs tethers the incoming vDNA to mitotic chromosomes to direct them into the nascent nuclei. To re-evaluate how dynamic L2 recruitment to cellular chromosomes occurs specifically during prometaphase, we developed a quantitative, microscopy-based assay for measuring the degree of chromosome recruitment of L2-EGFP. Analyzing various HPV16 L2 truncation-mutants revealed a central chromosome-binding region (CBR) of 147 amino acids that confers binding to mitotic chromosomes. Specific mutations of conserved motifs (IVAL286AAAA, RR302/5AA, and RTR313EEE) within the CBR interfered with chromosomal binding. Moreover, assembly-competent HPV16 containing the chromosome-binding deficient L2(RTR313EEE) or L2 (IVAL286AAAA) were inhibited for infection despite their ability to be transported to intracellular compartments. Since vDNA and L2 were not associated with mitotic chromosomes either, the infectivity was likely impaired by a defect in tethering of the vDNA to mitotic chromosomes. However, L2 mutations that abrogated chromatin association also compromised translocation of L2 across membranes of intracellular organelles. Thus, chromatin recruitment of L2 may in itself be a requirement for successful penetration of the limiting membrane thereby linking both processes mechanistically. Furthermore, we demonstrate that the association of L2 with mitotic chromosomes is conserved among the alpha, beta, gamma, and iota genera of Papillomaviridae. However, different binding patterns point to a certain variance amongst the different genera. Overall, our data suggest a common strategy among various PVs, in which a central region of L2 mediates tethering of vDNA to mitotic chromosomes during cell division thereby coordinating membrane translocation and delivery to daughter nuclei.


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