Attenuated Late-Phase Arc Transcription in the Dentate Gyrus of Mice Lacking Egr3
Lackie, Rachel E.
Elizalde, Diana I.
Grella, Stephanie L.
Damphousse, Chelsey C.
Gallitano, Amelia L.
Marrone, Diano F.
AffiliationUniv Arizona, Dept Basic Med Sci
Univ Arizona, McKnight Brain Inst
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CitationAttenuated Late-Phase Arc Transcription in the Dentate Gyrus of Mice Lacking Egr3 2017, 2017:1 Neural Plasticity
RightsCopyright © 2017 Amanda Maple et al. This is an open access article distributed under the Creative Commons Attribution License.
Collection InformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at firstname.lastname@example.org.
AbstractThe dentate gyrus (DG) engages in sustained Arc transcription for at least 8 hours following behavioral induction, and this time course may be functionally coupled to the unique role of the DG in hippocampus-dependent learning and memory. The factors that regulate long-term DG Arc expression, however, remain poorly understood. Animals lacking Egr(3) show less Arc expression following convulsive stimulation, but the effect of Egr3 ablation on behaviorally induced Arc remains unknown. To address this, Egr3(-/-) and wild-type (WT) mice explored novel spatial environments and were sacrificed either immediately or after 5, 60, 240, or 480 minutes, and Arc expression was quantified by fluorescence in situ hybridization. Although short-term (i.e., within 60 min) Arc expression was equivalent across genotypes, DG Arc expression was selectively reduced at 240 and 480 minutes in mice lacking Egr3. These data demonstrate the involvement of Egr3 in regulating the late protein-dependent phase of Arc expression in the DG.
VersionFinal published version
SponsorsNatural Sciences and Engineering Research Council of Canada; Ontario Mental Health Foundation; US National Institute of Mental Health Award [MH097803]; Science Foundation of Arizona Bisgrove Scholarship