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    Improved metabolism and redox state with a novel preservation solution: implications for donor lungs after cardiac death (DCD)

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    Author
    Schipper, David A.
    Louis, Anthony V.
    Dicken, Destiny S.
    Johnson, Kitsie
    Smolenski, Ryszard T.
    Black, Stephen M.
    Runyan, Ray
    Konhilas, John
    Garcia, Joe G.N.
    Khalpey, Zain
    Affiliation
    Univ Arizona, Coll Med, Dept Surg, Div Cardiothorac Surg
    Univ Arizona, Coll Med, Div Translat & Regenerat Med, Dept Med
    Univ Arizona, Hlth Sci Ctr
    Issue Date
    2017-05-24
    Keywords
    transplant
    metabolomics
    organ longevity
    
    Metadata
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    Publisher
    SAGE PUBLICATIONS INC
    Citation
    Improved metabolism and redox state with a novel preservation solution: implications for donor lungs after cardiac death (DCD) 2017, 7 (2):494 Pulmonary Circulation
    Journal
    Pulmonary Circulation
    Rights
    © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License.
    Collection Information
    This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
    Abstract
    Lungs donated after cardiac death (DCD) are an underutilized resource for a dwindling donor lung transplant pool. Our study investigates the potential of a novel preservation solution, Somah, to better preserve statically stored DCD lungs, for an extended time period, when compared to low-potassium dextran solution (LPD). We hypothesize that Somah is a metabolically superior organ preservation solution for hypothermic statically stored porcine DCD lungs, possibly improving lung transplant outcomes. Porcine DCD lungs (n = 3 per group) were flushed with and submerged in cold preservation solution. The lungs were stored up to 12 h, and samples were taken from lung tissue and the preservation medium throughout. Metabolomic and redox potential were analyzed using high performance liquid chromatography, mass spectrometry, and RedoxSYS (R), comparing substrate and pathway utilization in both preservation solutions. Glutathione reduction was seen in Somah but not in LPD during preservation. Carnitine, carnosine, and n-acetylcarnosine levels were elevated in the Somah medium compared with LPD throughout. Biopsies of Somah exposed lungs demonstrated similar trends after 2 h, up to 12 h. Adenosine gradually decreased in Somah medium over 12 h, but not in LPD. An inversely proportional increase in inosine was found in Somah. Higher oxidative stress levels were measured in LPD. Our study suggests suboptimal metabolic preservation in lungs stored in LPD. LPD had poor antioxidant potential, cytoprotection, and an insufficient redox potential. These findings may have immediate clinical implications for human organs; however, further investigation is needed to evaluate DCD lung preservation in Somah as a viable option for transplant.
    Note
    12 month embargo; First Published May 24, 2017
    ISSN
    2045-8932
    2045-8940
    PubMed ID
    28597777
    DOI
    10.1177/2045893217706065
    Version
    Final published version
    Sponsors
    US-Polish Fulbright Commission
    Additional Links
    http://journals.sagepub.com/doi/10.1177/2045893217706065
    ae974a485f413a2113503eed53cd6c53
    10.1177/2045893217706065
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